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TA Ferguson, H Kaplan, T Tezel, O Volpert, R Barriero, R Schadlu; Augmentation of Choroidal Neovascularization in a Laser Induced Mouse Model by PEDF . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2756.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Examine the effect of PEDF (Pigment epithelium derived growth factor) on laser induced choroidal neovascularization (CNV). Methods: Adult C57Bl/6 were anesthetized and 4 laser spots were placed in each quadrant of the fundus using a Krypton Red Laser (614 nm, 100µm, 0.5 sec, 200mW). Animals were treated with varying doses of PEDF administered using a mini osmotic pump implanted subcutaneously. Seven days following laser treatment mice were perfused with 3% FITC high molecular weight dextran, enucleated, and neovascularization analyzed by confocal microscopy. Data are recorded as incidence of CNV per laser spot, absolute area of the CNV complex, and relative area of CNV (which compares the area of CNV to the area of the laser spot). Results: Mice receiving lower doses of PEDF (totaling 45-90 µg over the experimental period) had significantly reduced incidences, absolute areas, and relative areas of CNV. Mice receiving higher doses of PEDF (300-400 µg) had significantly increased CNV. Intermediate doses (180-200 µg) of PEDF had no effect. Conclusion: PEDF demonstrates opposing effects on CNV. While low does can inhibit CNV, high does can increase the development of neovascularization. These results suggest the effects of PEDF on neovascularization are more complex than originally believed, and that caution should be exercised when PEDF therapies are considered.
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