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ME Boulton, J Cai, WG Jiang, A Ahmed; Role of Placenta Growth Factor (PlGF) and PlGF/VEGF Heterodimer in the Regulation of In Vitro Angiogenesis . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2762.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:To determine the role of placenta growth factor (PlGF) in the different stages of angiogenesis. Methods:Bovine retinal microvascular endothelial cells were isolated and grown in microvascular endothelial cell basal medium. We compared the effect of PlGF, VEGF and the VEGF/PlGF heterodimer at concentrations of 1 to 200ng on endothelial cell proliferation and migration. To assess the effect of the growth factors on tube formation cells were embedded in matrigel and tube length quantified by image analysis. Cell survival was assessed by the dye exclusion assay and Bcl-2 levels were determined by western blotting of protein extracts. Results:PlGF only weakly stimulated cell proliferation which was greatest for VEGF. PlGF had no effect on cell migration while the heterodimer showed activity intermediate between PlGF and VEGF. All three growth factors stimulated tube formation with the heterodimer demonstrating the greatest potency. Furthermore, PlGF and the heterodimer were able to sustain tube formation over a longer period than VEGF. PlGF, VEGF and VEGF/PlGF promoted cell survival equally in serum-depleted medium and was associated with Bcl-2 expression. PlGF and the heterodimer were able to sustain the survival effect for longer than VEGF alone. Conclusion:These findings indicate that PlGF and VEGF/PlGF heterodimer may play an important role in vessel stabilisation by regulating tube formation and cell survival.
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