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RS Jones, I D'Angelo, NC Brecha; AII Amacrine Cells Express Neuropeptide Y Receptor Y1 in the Rat and Monkey Retinas . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2773.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:In the mammalian retina, neuropeptide Y (NPY) is expressed primarily by wide field amacrine cells and displaced amacrine cells. These experiments investigated the localization of NPY Y1 receptors to AII amacrine cells in the rat and monkey retinas. The AII amacrine cell is a critical interneuron in the pathway for scotopic visual information in the retina. Methods:Retinas were obtained from adult Sprague Dawley rats and Macaque monkeys, fixed in 4% paraformaldehyde, and 14mm transverse sections were cut for immunohistochemistry. Polyclonal antibodies against a C-terminus fragment (amino acids 370-382) of the Y1 receptor and monoclonal antibodies against the calcium binding protein parvalbumin (PV) were used in these studies. Results:Y1 immunoreactivity in the rat retina was localized to the outer retina and puncta spanning strata 4 and 5 of the inner plexiform layer (IPL) in the inner retina. Immunoreactivity was absent from sections preincubated with Y1 peptide (amino acids 370-382). In the monkey retina, Y1 immunoreactivity was observed only in puncta spanning strata 4 and 5 of the IPL. Immunoreactivity was absent from sections preincubated with the peptide. Double-labeling immunohistochemistry with antibodies to PV, which predominantly label AII amacrine cells in the rat and monkey retinas, demonstrated that Y1 immunoreactive puncta are localized to AII amacrine cell processes in the IPL. Conclusion:Y1 localization on AII amacrine cell processes suggests that NPY likely modulates rod vision at this point by acting on Y1 receptors.
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