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X Zhang, C Friedman, AM Laties, CH Mitchell; Adenosine Attenuates the Effect of ATP P2X7 Agonist on Retinal Ganglion Cells . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2777.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To investigate the effect of ATP agonists and adenosine on intracellular Ca2+ levels of rat retinal ganglion cells (RGCs). Methods: Retinae were dissociated from Long Evan rat pups and cultured overnight. Cells were loaded with the dye fura-2 and Ca2+ levels in RGCs were measured ratiometrically using a fluorescent imaging system. Results: The Ca2+ of RGCs was elevated by a variety of purines; at 10µM, the efficacy ranking was BzATP≷UTP≷ATP. While Ca2+ returned to baseline after removal of ATP levels remained elevated after BzATP removal. Adenosine itself did not raise Ca2+ levels, but greatly attenuated the BzATP effect. When present ∼2 min before and during the BzATP application, adenosine reduced the peak Ca2+ significantly and the sustained elevation in Ca2+ concentration following BzATP was eliminated. Conclusion: The Ca2+ levels of rat RGCs can be elevated by ATP, UTP and BzATP but not by adenosine. The sustained elevation of Ca2+ after BzATP suggests that stimulation of the P2X7 receptor could damage cells. Adenosine may reduce this damage.
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