December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Regional Vulnerability of Axons in a Chronic Optic Nerve Ischemia Model in Primates
Author Affiliations & Notes
  • GA Cioffi
    Ophthalmology/Good Sam Hosp Devers Eye Institute Portland OR
  • L Wang
    Discoveries in Sight Portland OR
  • J Dong
    Discoveries in Sight Portland OR
  • G Cull
    Discoveries in Sight Portland OR
  • Footnotes
    Commercial Relationships   G.A. Cioffi, None; L. Wang, None; J. Dong, None; G. Cull, None. Grant Identification: NIH R01 05231 (GAC)
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2886. doi:
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      GA Cioffi, L Wang, J Dong, G Cull; Regional Vulnerability of Axons in a Chronic Optic Nerve Ischemia Model in Primates . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2886.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose: To study the regional pattern of optic nerve (ON) axonal damage induced by chronic ischemia in a primate model. Methods: Chronic ON ischemia (4-6 mths) was induced by delivering Endothelin-1 via a subcutaneous osmotic minipump and delivery tube to the supero-nasal retrobulbar space in one eye of 8 (5 are analyzed at time of submission) adult rhesus monkeys (contralateral eyes as controls). In vivo topographic changes of optic disc were evaluated with Heidelberg Retinal Tomography (HRT, 4 eyes) and stereophotography. Total axonal counts and regional densities of ON cross-sections (2 mm behind globe) were quantified with a novel automated image analysis system in 4 and 8 pie-shaped regions. Results: Compared to controls, there was significant axon loss (20-40%, P<0.01) in most regions of the ischemic eyes. However, the axonal loss was not uniform, with the nasal ON regions (close to the site of endothelin-1 delivery) suffering less damage (stat signif, see figure) compared to the temporal ON, in all five animals.  

Final HRTs, compared to baseline, in the ischemic eyes showed significant increase in the cup area, cup volume and decrease in rim volume and retinal nerve fiber layer thickness. Conclusion: Chronic ON ischemia selectively causes axonal damage of the temporal ON in a primate model. This suggests a regional vulnerability of the ON, with increased susceptibility of the temporal ON with a chronic ischemic insult.

Keywords: 448 ischemia • 456 lesion study • 506 pathology: experimental 

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