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SL Mills; Tracer Analysis Of Cone Coupling In The Rabbit Retina . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2919.
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Purpose: Mammalian cones are known to make gap junctions both with neighboring cones and also with rods. I injected tracers into individual cones of rabbit retina to see if coupling could be found and if there was colocalization with the connexin Cx35(36) Method: I isolated rabbit retinas and stained the cone population with peanut agglutinin-FITC. Single cones were filled with Neurobiotin and Lucifer Yellow. Some tissue was also stained with an antibody to Cx35. Results: Although total permeability between cones was small, lengthy perfusion times allowed coupling between cones to be clearly seen. Coupling to rods was never seen under these conditions, nor did injection into rods ever produce coupling to cones or other rods. The cone pedicles could be clearly visualized with either Lucifer Yellow or Neurobiotin-staining and revealed an extensive branching pattern of about 8 telodendria extending 1-1.5 microns beyond the pedicle. Many telodendria also divided into further branches. An antibody to Cx35 produced punctate staining along the telodendria. Conclusion: Although small, the gap junctions between cones are able to support passage of Neurobiotin. An individual cone pedicle is likely to be a very active site of gap junctions, based upon the number of telondendria and the presence of Cx35-staining along their processes.
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