December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Thrombospondin (TSP) Is the Primary Molecular Mediator of ACAID-Inducing Properties of TGFß2-Treated Antigen Presenting Cells (APC)
Author Affiliations & Notes
  • S Masli
    Dept of Ophthalmology Harvard Medical School
    Schepens Eye Research Institute Boston MA
  • B Turpie
    Schepens Eye Research Institute Boston MA
  • JW Streilein
    Dept of Ophthalmology Harvard Medical School
    Schepens Eye Research Institute Boston MA
  • Footnotes
    Commercial Relationships   S. Masli, None; B. Turpie, None; J.W. Streilein, None. Grant Identification: NIH EY05678
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2958. doi:
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      S Masli, B Turpie, JW Streilein; Thrombospondin (TSP) Is the Primary Molecular Mediator of ACAID-Inducing Properties of TGFß2-Treated Antigen Presenting Cells (APC) . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2958.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose:Exposure of APCs to TGFß2 up-regulates several immunomodulatory genes: TGFß, thrombospondin, IFNα/ß. We wished to determine if one of these gene products serves as the major molecular mediator of the ACAID-promoting effects of TGFß2. Methods:Macrophage hybridoma #59 cells were pulsed with ovalbumin (OVA) and cultured in the presence of TGFß2, TSP, or IFNß. The treated cells were examined (a) in vitro by RT-PCR, for expression of genes known to be up-regulated by TGFß2 treatment: TSP, MIP-2, TGFß, IFNα, IFNß, IFNα/ßReceptor and flow cytometry for TSP receptors (CD47, CD36); and (b) in vivo for their capacity to suppress OVA-specific delayed hypersensitivity (DH) when injected into naïve mice immunized subsequently with OVA plus complete Freunds' adjuvant. Results:APCs treated with TSP or TGFß2, but not with IFNß, impaired DH responses in vivo. TSP-treated APCs displayed enhanced expression of MIP-2, TGFß similar to TGFß2-treatment, whereas IFNß-treated APCs displayed suppressed expression of these same genes that are known to be important in ACAID induction. Conclusion:TSP acts as a surrogate for TGFß2 in conferring ACAID-inducing properties on APCs. Since it is a very early response gene after TGFß2 treatment, TSP appears to be the major mediator of the ACAID-promoting activities of TGFß2.

Keywords: 301 ACAID • 320 antigen presentation/processing • 435 immunomodulation/immunoregulation 
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