December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Cellular Pathways in ACAID: Evidence That Thymic NK T Cells Induce Splenic Regulatory T Lymphocytes
Author Affiliations & Notes
  • RE Cone
    Pathology/Vision Immunology Center University of CT Health Center Farmington CT
  • Y Wang
    Pathology/Vision Immunology Center
    University of Connecticut Health Center Farmington CT
  • DL Foss
    Pathology
    University of Connecticut Health Center Farmington CT
  • J O'Rourke
    Pathology/Vision Immunology Center
    University of Connecticut Health Center Farmington CT
  • I Goldschneider
    Pathology
    University of Connecticut Health Center Farmington CT
  • Footnotes
    Commercial Relationships   R.E. Cone, None; Y. Wang, None; D.L. Foss, None; J. O'Rourke, None; I. Goldschneider, None. Grant Identification: NIH Grant EY 13243, AI 49882, CT Lions Eye Research Foundation
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2963. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      RE Cone, Y Wang, DL Foss, J O'Rourke, I Goldschneider; Cellular Pathways in ACAID: Evidence That Thymic NK T Cells Induce Splenic Regulatory T Lymphocytes . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2963.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: Intracameral injection of antigen induces the appearance of antigen-specific, CD4-,CD8-, NK1.1+ thymocytes (AC-THY) that adoptively transfer Anterior Chamber-Associated Immune Deviation (ACAID). Recipients of AC-THY generate antigen-specific splenic T cells (AC-SPL) that induce or effect the regulation of delayed-type hypersensitivity (DTH). Here we used adoptive transfer assays to determine whether the AC-THY induce and/or generate splenic CD4+ and CD8+ (inducer, effector respectively) regulatory T cells. Methods: AC-THY and AC-SPL were obtained from Balb/c, C57Bl/6 or AKR X C57Bl/6 mice that received intracameral TNP-BSA or BSA or AC-THY and TNP-BSA + Freund's Complete Adjuvant sc. Mononuclear cells obtained from the peripheral blood of AC-injected transgenic GFP (C57Bl/6) mice 24hr after injection of TNP-BSA were injected iv into C57Bl/6, GFP- mice. The phenotype of GFP+ cells in the spleens and thymus of recipients was determined with phycoerythrin-monoclonal anti-F4/80 and anti-CD11c antibodies. The immunoregulatory effector activity of AC-SPL was determined by injection of AC-SPL into the footpad of TNP-BSA-senstized mice concurrent with epicutaneous picryl chloride (PCl). The afferent immunoregulatory activity of AC-SPL was determined by iv injection of AC-SPL into TNP-BSA-senstitized mice and epicutaneous challenge with PCL 1 week later. Monoclonal anti-LY5.1 or 5.2 antibodies and immunomagnetic beads were used to separate LY 5.1 and LY 5.2+ AC-SPL from LY 5.2+ recipients of LY 5.1+ AC-THY. Results: F4/80+,CD11c+ (dendritic) peripheral blood mononuclear cells from transgenic GFP mice receiving intracameral TNP-BSA were thymus seeking when injected into C57Bl/6 mice and F4/80+, CD11c- peripheral blood monoculear cells from AC-injected mice were spleen seeking. The recipients of AC-THY induced by homologous antigen produced CD8+ splenic immunoregulatory effector cells. LY5.2+ (C57Bl/6) TNP-BSA-sensitized recipients of Ly5.1+AC-THY (AKR X C57Bl/6) produced LY5.1-,Ly5.2+ splenic immunoregultory effector and inducer cells. Conclusion: The results support our belief that (i) in ACAID intracameral injection of antigen induces blood-borne dendritic cells that deliver antigen to the thymus (ii) thereby inducing thymic NK T cells that migrate to the spleen (iii) where they activate afferent and efferent immunoregulatory splenic T cells that were induced by systemic immunization.

Keywords: 301 ACAID • 435 immunomodulation/immunoregulation • 433 immune tolerance/privilege 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×