December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Contact Lens Solution Efficacy against Acanthamoeba castellani
Author Affiliations & Notes
  • MF Mowrey-McKee
    Toxicology / Microbiology CIBA Vision Corporation Duluth GA
  • Footnotes
    Commercial Relationships    M.F. Mowrey-McKee, CIBA Vision Corporation E. Grant Identification: none
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3084. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      MF Mowrey-McKee; Contact Lens Solution Efficacy against Acanthamoeba castellani . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3084.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Abstract: : Purpose: Five competitive contact lens care products were tested for amoebicidal efficacy using Acanthamoeba castellani, ATCC 30234, (cysts and trophozoites) after a 6 hour exposure. Methods: The study was conducted at Bio-Concept Laboratories, Inc. Trophozoite cultures were prepared in vented tissue culture flasks containing PYG broth by incubation at 35+1º C for 11 days. Cyst suspensions were prepared by incubation of trophozoites in PBS plus heat killed yeast on Page's Saline Agar plates at 35+1º C for 14 days. The lens care products were inoculated in triplicate using their respective lens cases. After the 6 hour exposure aliquots of the challenged solutions were transferred to Dey/Engley Broth (DEB) and then further diluted in PYG broth. Microtiter plates were used for the higher dilutions. Flasks and plates were incubated for 14 days at 35+1º C and were examined with an inverted light microscope at days 7 and 14 for the presence of viable trophozoites. Results: The following log reductions were observed for the cysts: 2.0 for BLUE Vision/BLUE SEPTTM, 1.8 for AOSEPT® Plus/Clear CareTM, 0.7 for Oxysept 1step®, 0.5 for Opti-Free® Express® with Aldox®, and 0.2 for easyvision one step+. The following log reductions were observed for the trophozoites: 2.7 for BLUE Vision/BLUE SEPTTM, 2.2 for AOSEPT® Plus/Clear CareTM, 2.5 for Oxysept 1step®, 2.5 for Opti-Free® Express® with Aldox® and 1.8 for easyvision one step+. Conclusion: Only BLUE Vision/BLUE SEPTTM and AOSEPT® Plus/Clear CareTM exhibited significant antimicrobial activity against the cyst form of Acanthamoeba castellani. Oxysept 1step® showed mild activity against the cysts and easyvision one step+ and Opti-Free® Express® with Aldox® showed virtually no anti-acanthamoeba activity against the cyst form following 6 hours of exposure.

Keywords: 302 Acanthamoeba • 367 contact lens 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.