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MF Mowrey-McKee; Contact Lens Solution Efficacy against Acanthamoeba castellani . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3084.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Five competitive contact lens care products were tested for amoebicidal efficacy using Acanthamoeba castellani, ATCC 30234, (cysts and trophozoites) after a 6 hour exposure. Methods: The study was conducted at Bio-Concept Laboratories, Inc. Trophozoite cultures were prepared in vented tissue culture flasks containing PYG broth by incubation at 35+1º C for 11 days. Cyst suspensions were prepared by incubation of trophozoites in PBS plus heat killed yeast on Page's Saline Agar plates at 35+1º C for 14 days. The lens care products were inoculated in triplicate using their respective lens cases. After the 6 hour exposure aliquots of the challenged solutions were transferred to Dey/Engley Broth (DEB) and then further diluted in PYG broth. Microtiter plates were used for the higher dilutions. Flasks and plates were incubated for 14 days at 35+1º C and were examined with an inverted light microscope at days 7 and 14 for the presence of viable trophozoites. Results: The following log reductions were observed for the cysts: 2.0 for BLUE Vision/BLUE SEPTTM, 1.8 for AOSEPT® Plus/Clear CareTM, 0.7 for Oxysept 1step®, 0.5 for Opti-Free® Express® with Aldox®, and 0.2 for easyvision one step+. The following log reductions were observed for the trophozoites: 2.7 for BLUE Vision/BLUE SEPTTM, 2.2 for AOSEPT® Plus/Clear CareTM, 2.5 for Oxysept 1step®, 2.5 for Opti-Free® Express® with Aldox® and 1.8 for easyvision one step+. Conclusion: Only BLUE Vision/BLUE SEPTTM and AOSEPT® Plus/Clear CareTM exhibited significant antimicrobial activity against the cyst form of Acanthamoeba castellani. Oxysept 1step® showed mild activity against the cysts and easyvision one step+ and Opti-Free® Express® with Aldox® showed virtually no anti-acanthamoeba activity against the cyst form following 6 hours of exposure.
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