Purchase this article with an account.
T Fujihara, T Nagano, T Murakami, M Nakamura, K Nakata; P2Y2 Agonist, INS365 Suppresses Loss of Corneal Epithelial Integrity in a Rabbit Short-term Dry Eye Model . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3114.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: As the purinoceptor P2Y2 agonist, INS365, stimulates both fluid transport across rabbit conjunctival tissue and mucin secretion from goblet cells, it is a candidate for dry eye therapy. Therefore, we sought to determine in a short-term rabbit dry eye model whether INS365 containing eye drops could suppress any of the epithelial damage produced by blockage of the blink reflex. Methods: Five min after anesthetization with urethane, either 50 µL of saline or INS365 containing eyedrops were applied to eyes. Rabbits were allowed to blink once 5 min post-application. In the dose response study employing 0.001--1.0% INS365, the eyes were kept open for 3 h. With the time course study, they were kept open from 1 to 3 h. Eyes were extirpated at appropriate times and corneal epithelial integrity was evaluated based on the extent of methylene blue staining in histological sections. Results: After 3 h of desiccation, the amount of extractable dye following sacrifice increased by more than 4-fold in the vehicle-treated eyes. However, in those rabbits treated with 1.0% INS365, dye recovery was invariant. From 0.001% to 1.0% INS365, the relative decreases in staining were concentration dependent. Such decreases were in all cases significant at concentrations greater than 0.1%. Conclusion: These results suggest that INS365 containing eye drops may be of therapeutic value in decreasing the loss of corneal epithelial integrity in dry eye.
This PDF is available to Subscribers Only