December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Effect of Inflammatory Cytokines on the Regulation of Muc5ac Gene Expression
I-J Wang; C-Y Wu; F-R Hu; P-T Hung
Author Affiliations & Notes
  • I-J Wang
    Ophthalmology National Taiwan Univ Hosp Taipei Taiwan Republic of China
  • C-Y Wu
    Ophthalmology Far Eastern Memorial Hospital Taipei Taiwan Republic of China
  • F-R Hu
    Ophthalmology National Taiwan Unversity Hospital Taipei Taiwan Republic of China
  • P-T Hung
    Ophthalmology National Taiwan Unversity Hospital Taipei Taiwan Republic of China
  • Footnotes
    Commercial Relationships   I. Wang, None; C. Wu, None; F. Hu, None; P. Hung, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3157. doi:
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      I-J Wang, C-Y Wu, F-R Hu, P-T Hung; Effect of Inflammatory Cytokines on the Regulation of Muc5ac Gene Expression . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3157.

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Abstract

Abstract: : Purpose:To investigate the regulation of Muc5AC gene expression by different kind of cytokines. Methods:Human Muc5AC promoter with a luciferase reporter gene was transfected into a human conjunctival epithelial cell by lipofectin. Then,the promoter activity was challenged with IL-1b, IL-2, IL-6, IL-8 and TNF-a respectively. In vivo assay of Muc5AC was performed by gene gun delivery of plasmid-coated gold particles into rabbit conjunctiva. Results:The Muc5AC reporter activity could be affected by these pro-inflammatory cytokines in vitro and in vivo.Stimulation of transfected human conjunctival cell line with TNF-a and IL-1b resulted in a two-fold and four- to sixfold increase in Muc5AC promoter activity respectively. In vivo assay also revealed a several-fold increase in Muc5AC promoter activity in transtected rabbit conjunctival tissue after being challenged with different cytokines,especially with TNF-a, IL-2 and IL-8. Conclusion:The secretion of secretary mucin 5 was affected by inflammatory cytokines. Our results imply that decreasing the ocular surface inflammation may play an important role in the treatment of ocular surface disorder, such as dry eye syndrome.

Keywords: 417 gene/expression • 380 cytokines/chemokines • 365 conjunctiva 
© 2002, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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