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N Asano-Kato, N Okada, K Fukagawa, Y Takano, T Kawakita, M Tanaka, K Tsubota, H Fujishima; VEGF Production From Cultured Conjunctival Fibroblasts Stimulated by Th2 Cytokines . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3167.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Formation of giant papillae leading to tissue remodelling is a characteristic of severe allergic conjunctival disease. Neovascularization is an important feature of such remodelling. In this study, we examined production of vascular endothelial growth factor (VEGF) from cultured conjunctival fibroblasts stimulated with Th2 cytokines. Methods:Conjunctival fibroblasts were cultured in DMEM/F12 medium at 37C for 24 hours, and were then stimulated with IL-4, TNF-alpha, IFN-gamma, IL-13 and eosinophil lysate. Concentrations of VEGF in the supernatant were measured by ELISA. Results:The concentration of VEGF in the control culture was 276.9±132.2 pg/ml. IL-4, IL-13 and eosinophil lysate increased VEGF production from cultured fibroblasts (63%, 84%, and 308%, respectively). TNF-alpha and IFN-gamma did not upregulate VEGF production. Conclusion:VEGF production in cultured conjunctival fibroblasts was significantly increased by IL-4, IL-13 and eosinophil lysate. Th2 cytokines and proteins released from eosinophils may play a crucial role in the neovascularization in giant papillae.
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