December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Distribution of Organic Anion Transporting Polypeptide (oatp-E) in Rat Eye
Author Affiliations & Notes
  • A Ito
    Ophthalmology Tohoku University Sch of Med Sendai Japan
  • K Yamaguchi
    Tohoku University School of Medicine Sendai Japan
  • T Abe
    2 Division of Nephrology Endocrinology and Vascular medicine Department of Medicine
    Tohoku University School of Medicine Sendai Japan
  • M Tamai
    Tohoku University School of Medicine Sendai Japan
  • Footnotes
    Commercial Relationships   A. Ito, None; K. Yamaguchi, None; T. Abe, None; M. Tamai, None. Grant Identification: The Ministry of Education, Science, Sports and Culture
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3239. doi:
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      A Ito, K Yamaguchi, T Abe, M Tamai; Distribution of Organic Anion Transporting Polypeptide (oatp-E) in Rat Eye . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3239.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose: The aim of this study is to examine the expression of a novel multifunctional Na+-independent organic anion transporting polypeptide, termed oatp-E involving the transport of thyroid hormone in rat eye at protein level and mRNA level. Methods: Quantitative reverse transcription-polymerase chain reaction was performed by using gene specific primer. Western blot analysis and immunohistochemistry were also performed by raising specific antibody against oatp-E. Result: By quantitative reverse transcription-polymerase chain reaction, oatp-E was expressed in rat retina, cornea, and ciliary body /iris in order. Western blot analysis detected a single band in rat retina. It was revealed the specificity of the oatp-E antibody. The oatp-E immunostaining was mainly detected in the cornal epithelium, pigment and nonpigment epithelium of the ciliary body and iris. In the retina, intense immunostaining was detected in the inner and outer nuclear layer, ganglion cell layer and nerve fiber layer, and weak immunostaining was also seen in the retinal pigment epithelium. Conclusion: These results demonstrate that oatp-E is localized mainly in the cornea, ciliary body, iris and retina, suggesting a functional role for organic anion transport in this specialized ocular tissues. In conclusion, these data suggest that oatp-E may be involved in the transport of thyroid hormone in the rat eye.

Keywords: 372 cornea: epithelium • 348 ciliary body • 554 retina 

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