December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Persistent Elevation of Intaocular Pressure in Mouse
Author Affiliations & Notes
  • M Aihara
    Glaucoma Center University of California San Diego La Jolla CA
  • JD Lindsey
    Glaucoma Center University of California San Diego La Jolla CA
  • RN Weinreb
    Glaucoma Center University of California San Diego La Jolla CA
  • Footnotes
    Commercial Relationships   M. Aihara, None; J.D. Lindsey, None; R.N. Weinreb, None. Grant Identification: Support: NEI EY05990 and Joseph Drown Foundation
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3278. doi:
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      M Aihara, JD Lindsey, RN Weinreb; Persistent Elevation of Intaocular Pressure in Mouse . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3278.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose: Persistent elevation of intraocular pressure (IOP) in the mouse eye may be useful for investigation of the mechanism of glaucoma in mutant and transgenic mice. Methods: Our approach was to obstruct the conventional outflow pathway by laser photocoagulation. One eye in each of 22 black Swiss mice was dilated by topical treatments with mydriatic agents. After ketamine/xylazine anesthesia, the anterior chamber of the dilated eye was flattened by aspiration of aqueous fluid with a microneedle. Next, peri-limbal laser photocoagulation (532 nm) was performed. The duration, power and spot size were 0.05 seconds, 0.1watt, and 200µm. After laser treatment, IOP of the treated eye (Ttx) and non-treated eye (Tc) were measured by a microneedle and pressure transducer method every week for a month. Results: The evaluation of IOP increase was calculated by the following formula; IOP increase(%)=100(Ttx-Tc)/Tc. Over 30% increase was defined as success of treatment. In one week, 15/22 (68%) eyes had successful increase of IOP (mean IOP increase: 91 ± 39%). After 4 weeks, 9/21 (41%) eyes maintained high pressure and the mean increase was 62 ± 17%. Conclusion: This appears to be a promising method to induce persistent IOP elevation in the mouse eye.

Keywords: 316 animal model • 444 intraocular pressure • 503 outflow: trabecular meshwork 

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