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WM Peterson, CH Meyer, LB Oliveira, KP Winter, CA Toth; INS37217, a P2Y2 Receptor Agonist, Increases Subretinal Fluid Reabsorption in a Porcine Model of Limited Macular Translocation Surgery . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3513.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: The synthetic P2Y2 receptor agonist INS37217 has been shown to enhance subretinal fluid reabsorption in rat and rabbit models of small retinal detachments, presumably by activating the RPE pump. The present study is designed to evaluate the effects of INS37217 in stimulating reattachment of large bullous retinal detachments produced in a pig model of limited macular translocation surgery. Methods: In the first series of experiments, ∼5 µl of balanced salt saline plus (BSS+) was injected subretinally into both eyes, followed by a masked intravitreal BSS+ injection (50 µl) with or without INS37217 in 3-10 week old pigs. Subretinal fluid reabsorption was observed over 5 hr using indirect ophthalmoscopy. In a second series of experiments, a central vitrectomy and a large retinal detachment extending 1-2 quadrants (300 µl of BSS+ injected subretinally) were performed in one eye in each of 12 pigs. Either BSS+ without or with INS37217 was injected into the midvitreous cavity and observed over 3-7 days by indirect ophthalmoscopy, ultrasound and OCT-imaging. Results: Enhanced reabsorption of subretinal fluid was observed in eyes treated with INS37217 in both series of experiments. In the second series of experiments, complete reabsorption of subretinal fluid in the large retinal detachments was observed within 36 hr in all eyes treated with INS37217, and after 4-5 days in control eyes. Conclusion: Intravitreal delivery of INS37217 stimulated reattachment of large retinal detachments produced in the context of limited macular translocation surgery in the young pig. INS37217 may therefore be useful as a surgical adjunct to stimulate subretinal fluid reabsorption in retinal detachment or in limited macular translocation surgery in humans.
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