December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Rapid Progesterone Actions on Bovine Lens Epithelial Cells
Author Affiliations & Notes
  • AK Samadi
    Biochemistry Kirksville Coll Osteopathic Med Kirksville MO
  • A Gueorguiev
    Kirksville College of Osteopathic Medicine Kirksville MO
  • G Carlson
    Kirksville College of Osteopathic Medicine Kirksville MO
  • R Cenedella
    Kirksville College of Osteopathic Medicine Kirksville MO
  • Footnotes
    Commercial Relationships   A.K. Samadi, None; A. Gueorguiev, None; G. Carlson, None; R. Cenedella, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3540. doi:
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      AK Samadi, A Gueorguiev, G Carlson, R Cenedella; Rapid Progesterone Actions on Bovine Lens Epithelial Cells . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3540.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose: Membrane steroid binding protein, which we identified in lens epithelial cells, is hypothesized to mediate a rapid effect of progesterone on lens calcium flux. The present result tests this hypothesis. Methods: We studied the effect of progesterone on Ca2+ signaling in in situ lens epithelial cells loaded with Fura-2 and fluorescence imaging system to evaluate the short-term effect of progesterone. The effect of progesterone on the calcium channel receptors was studied using Mn quenching technique. Results: Progesterone (10-100uM) induced a transient increase in [Ca2+]i within seconds, which is due to Ca2+ influx from the extracellular milieu, as shown by the effects of calcium chelator EGTA . Progesterone has no significant effect on the store-mediated release in the absence of extracellular Ca2+. Progesterone inhibited the thapsigargin-induced sustained [Ca2+]i elevation. It appears that progesterone reduces thapsigargin mediated calcium influx. A Mn2+-induced decline of fluorescent intensity at 360nm excitation was accelerated. However, in some cells, there was a transient decline in the rate of Mn2+ quench rate. Conclusion: This is the first report of a nongenomic rapid effects of a steroid hormone on lens cells and specifically on the intracellular concentration of calcium in lens epithelial cells. These data indicate a novel mode of direct action of progesterone on lens epithelial cells, which is not mediated through the classical nuclear receptor response, but likely through plasma membrane receptors.

Keywords: 338 cataract • 334 calcium • 541 receptors: pharmacology/physiology 

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