December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Effect of Insulin on Epithelial-Mesenchyme Transition(EMT) Iinduced by TGF-beta in Bovine Lens Explants
H Choi; J-T Kim; C-K Joo
Author Affiliations & Notes
  • H Choi
    Laboratory of Ophthalmology and Visual Science Catholic Medical Univ Seoul Republic of Korea
  • J-T Kim
    Laboratory of Ophthalmology and Visual Science Catholic Medical University Seoul Republic of Korea
  • C-K Joo
    Laboratory of Ophthalmology and Visual Science Catholic Medical University Seoul Republic of Korea
  • Footnotes
    Commercial Relationships   H. Choi, None; J. Kim, None; C. Joo, None. Grant Identification: Support: MOST Grant 00-J-LF-01-B-78
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3545. doi:
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      H Choi, J-T Kim, C-K Joo; Effect of Insulin on Epithelial-Mesenchyme Transition(EMT) Iinduced by TGF-beta in Bovine Lens Explants . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3545.

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Abstract

Abstract: : Purpose: Epithelial-mesenchyme transition(EMT) is a dynamic process in which epithelial cells transit to mesenchymal cells. Transforming growth factor (TGF)-beta is a multifunctional cytokine, that is well known to induce EMT. Our previous studies showed that TGF-ß induced the expression of mesenchymal marker in lens epithelial cells (LECs). The purpose of this study is to examine a role of insulin in EMT of bovine lens induced by TGF-beta. Methods: We cultured bovine lens epithelial explants in MEM containing 0.2% bovine serum albumin at 37°C for 12 hrs and treated them with 10ng/ml TGF-beta or 100ng/ml insulin for 24hrs. For analysis of extracellular matrix (ECM) protein and alpha-smooth muscle actin (SMA) we utilized reverse transcription-polymerase chain reaction (RT-PCR) and western blot analyses. Results: The protein and RNA levels of fibronectin and alpha-SMA were increased after TGF-beta treatment, whereas these increases were inhibited by insulin. Conclusion: Insulin may play a role of inhibition of EMT induced by TGF-beta.

Keywords: 522 posterior capsular opacification (PCO) • 423 growth factors/growth factor receptors • 403 extracellular matrix 
© 2002, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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