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K Katsumura, M Kobayashi, T Ikeda; Effect of Transforming Growth Factor Beta and Tranilast to Cultured Human Lens Epithelial Cell . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3548.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:In after cataract, lens epithelial cells undergo proliferation, metaplasia, and collagen synthesis. The transforming growth factor beta (TGF beta) is said to promote synthesis of collagen. Otherwise, tranilast , which is used for allergic disease has been known to inhibit proliferation of fibroblast and synthesis of TGF beta. In this study, we examined whether TGF beta and tranilast affect the proliferation of cultured human lens epithelial cells and their production of collagen in vitro. Methods:Human lens epithelial cells were obtained from the lens anterior capsule excised during cataract surgery. After harvesting in 96-well microplates in various concentrations of TGF beta for a week, we measured cell number using WST-8 KIT (DOJIN CHEMICAL) and the production of collagen type I and IV using cell-captured enzyme immunoassay (CC-EIA). In the presence of optimal concentration of TGF beta, we measured the number of LEC and the amount of produced collagen type I and IV as described above. Results:The production of collagen type I and IV in the presence of 5ng/ml TGF was significantly increased than that of control, however the cell number did not show any difference between two groups. Cultured lens epithelium cells under 5ng TGF+200µg/ml tranilast did not proliferate and showed no production of collagen type I and IV. Conclusion:Tranilast can reduce the proliferation of lens epithelial cells and their collagen production, which might be effective for the inhibition of secondary fibrosis after cataract surgery.
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