December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Morphological Characterization of the alphaA-/ alphaB-Crystallin Double Knockout Mouse Lens
Author Affiliations & Notes
  • DL Boyle
    National Eye Institute NIH Bethesda MD$; Genetic Therapy Inc Gaithersburg; Division of Biology Kansas State University Manhattan KS
  • L Takemoto
    Manhattan KS
  • JP Brady
    Manhattan KS
  • EF Wawrousek
    Manhattan KS
  • Footnotes
    Commercial Relationships   D.L. Boyle, None; L. Takemoto, None; J.P. Brady, None; E.F. Wawrousek , None. Grant Identification: Supported in part by NIH Grant EY02932
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3582. doi:
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      DL Boyle, L Takemoto, JP Brady, EF Wawrousek; Morphological Characterization of the alphaA-/ alphaB-Crystallin Double Knockout Mouse Lens . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3582.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose: Characterize the gross lenticular morphology, by scanning electron microscopy (SEM) and confocal microscopy, of normal mice and mice with targeted disruption alphaA- and alphaB-crystallin genes. Methods: Lenses from 129SvEvTac mice (wild type) and mice with the targeted disruption of alphaA- and alphaB-crystallin genes were examined by standard scanning electron microscopy and confocal microscopy. Results: Equatorial and axial (sagittal) dimensions of lenses for alphaA/BKO mice were significantly smaller than age-matched wild type lenses. No posterior sutures or fiber cells extending to the posterior capsule were found in alphaA/BKO lenses. Aberrant nucleic acid staining was observed in the posterior subcapsular region of 5 wk and anterior subcapsular cortex of 54 wk alphaA/BKO lenses. Gross morphological differences were observed in the equatorial, posterior and anterior regions of lenses from alphaA/BKO mice as compared to wild type mice. Conclusions: The results, in conjunction with previous studies (Brady et al., 2001), indicate that predominately alphaA-crystallin, and to a lesser extent, alphaB-crystallin, are necessary for lens transparency, and may be essential for proper fiber cell formation.

Keywords: 378 crystallins • 315 anatomy • 343 chaperones 

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