December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Metabolic Transformations of Carotenoids in Ocular Tissues of Humans and Non-Primate Animal Models
Author Affiliations & Notes
  • PS Bernstein
    Moran Eye Center University Of Utah Salt Lake City UT
  • D-Y Zhao
    Moran Eye Center University Of Utah Salt Lake City UT
  • F de Moura
    Chemistry and Biochemistry University of Maryland College Park MD
  • C Aebischer
    Vitamins and Fine Chemicals Hoffmann-La Roche Basel Switzerland
  • F Khachik
    Chemistry and Biochemistry University of Maryland College Park MD
  • Footnotes
    Commercial Relationships    P.S. Bernstein, Kemin Foods F; D. Zhao, None; F. de Moura, None; C. Aebischer, Hoffmann-La Roche E; F. Khachik, None. Grant Identification: Support: NIH Grant EY11600, Kemin Foods, and Research to Prevent Blindness
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3603. doi:
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      PS Bernstein, D-Y Zhao, F de Moura, C Aebischer, F Khachik; Metabolic Transformations of Carotenoids in Ocular Tissues of Humans and Non-Primate Animal Models . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3603.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose:To determine the absolute configuration and stereochemistry of xanthophyll carotenoids in human, quail, and frog ocular tissues in comparison to serum and liver and to elucidate the most likely pathways for metabolism of dietary (3R,3'R,6'R)-lutein and (3R,3'R)-zeaxanthin in the eye. Methods:Configurational isomers of carotenoids and their metabolites from pooled human, quail, and frog serum, liver, retina, RPE/choroid, ciliary body, iris, and lens were extracted, identified, and quantified by reverse-phase, normal phase, and chiral HPLC. Results:(3R,3'S;meso)-Zeaxanthin, a nondietary carotenoid, was abundant in human macula and retina, but was undetectable in liver and serum. Quails and frogs exhibited comparable ocular specific presence of (3R,3'S;meso)-zeaxanthin in their retinal tissues. Other carotenoid metabolites such as (3R,3'S,6'R)-lutein (3'-epilutein) and 3-hydroxy-ß ,ϵ-carotene-3'-one were also ocular specific, but to a lesser extent. Conclusion:The most likely metabolic transformations of carotenoids in the human eye involve a series of oxidation-reduction and double-bond isomerization reactions. Quails and frogs appear to possess similar biochemical pathways for conversion of dietary (3R,3'R,6'R)-lutein and (3R,3'R)-zeaxanthin to metabolites observed in humans and thus may serve as excellent nonprimate animal models for metabolic studies and for the isolation of the relevant enzymes.

Keywords: 337 carotenoids/carotenoid binding proteins • 467 metabolism • 316 animal model 
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