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GJ Ko, Q Chu, S Garg, R Sanchez, AA Sadun, J Sebag, TT Lam; Neuron-Specific Enolase in Cell Damage after Retinal Ischemia-Reperfusion Injury in Rats . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3614.
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Purpose:Neuron-specific enolase (NSE), a neuron and neuroendocrine cell-specific glycolytic enzyme, is released into the serum, the CSF and other tissues following neuronal injury. This study was conducted to investigate whether tissue and/or serum levels of NSE may be indicators of inner retinal degeneration after retinal ischemia-reperfusion injury in rats. Methods:Intraocular pressure was maintained for 60 minutes at 110mm Hg in anesthetized male albino Lewis rats. Retinal and blood samples were obtained at 0, 2, 4, 18, 24, 48 and 72 hours after the insult. Dot-blot and Western analysis were performed using a monoclonal mouse anti-human neuron-specific enolase (dot-blot: 1:500; Western: 1:200) (DAKO Corp., Carpinteria, CA). Immunohistochemistry was used to localize NSE in retinal sections. Results:Retinal NSE levels increased at 0, 2 and 4 hours after the insult, reached maximal levels from 18 to 24 hours, and decreased from 48 to 72 hours. Serum NSE levels also increased gradually, reaching maximal levels from 8 to 18 hours and showing noticeably elevated levels even at 72 hours. Western blotting confirmed the detection of the NSE gamma subunit detected at 46 kDa. Immunohistochemistry showed mild NSE immunoreactivity (IM) in the retina except the inner and outer segments with moderate IM in the cell bodies of scattered cells in the retinal ganglion cell layer (RGCL) and the inner nuclear layer (INL) in the normal retinas. After the insult and up to 48 hours, intense IM was noted in the inner limiting membrane (ILM), the nerve fiber layer (NFL) and RGCL. Conclusion: Both tissue and serum NSE levels are reliable indicators of inner retinal cell damage.
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