December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
The Intrinsic Renin-Angiotensin System (RAS) Of The Retina
Author Affiliations & Notes
  • TH Wheeler-Schilling
    Research Department University Eye Hospital Tuebingen Tuebingen Germany
  • E Zrenner
    Research Department University Eye Hospital Tuebingen Tuebingen Germany
  • E Guenther
    Research Department University Eye Hospital Tuebingen Tuebingen Germany
  • K Kohler
    Research Department University Eye Hospital Tuebingen Tuebingen Germany
  • Footnotes
    Commercial Relationships   T.H. Wheeler-Schilling, None; E. Zrenner, None; E. Guenther, None; K. Kohler, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3626. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      TH Wheeler-Schilling, E Zrenner, E Guenther, K Kohler; The Intrinsic Renin-Angiotensin System (RAS) Of The Retina . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3626.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Abstract: : Purpose: Recent studies imply a possible contribution of the RAS and its principal effector- and receptor molecules to the neurotransmission in the retina. Purpose of this study is to show the local existence of members of the RAS cascade (renin cleaves its substrate angiotensinogen (AGT) to form angiotensin I (AngI); AngI is converted by angiotensin converting enzyme (ACE) to angiotensin II (AngII)) and its receptors, which are mediated by two major subtypes AT1 and AT2, in the retina of various species and different developmental stages. Methods: Reverse transcription polymerase chain reaction (RT-PCR) was used to detect mRNAs for renin, AGT, ACE, and its receptors AT1 and AT2. In situ-hybridization (ISH) for the detection of renin and AT2-mRNA was applied. For the cellular localisation of the proteins by immunohistochemistry antibodies directed against renin, AngII, ACE, AT1 and AT2 were used. Additionally, we took advantage of a radioimmunoassay to detect the AngII-content. Results: The mRNAs for renin, AGT, ACE, AT1 and AT2 are expressed in the retina. Immunohistochemistry for AngII revealed labelled amacrine cells in the inner nuclear layer (INL) and fibers in sublayer 3 and 5 of the inner plexiform layer (IPL). ACE immunoreactive cells were detected in the INL and ganglion cell layer (GCL). A specific AT2 staining was observed in the GCL. In the INL horizontal cells and amacrine cells were stained at early stages (P3-P16) only. ISH for renin showed a labelling of the majority of cells in the GCL and less intense in the INL. Taken the level of blood-borne AngII as a baseline, the AngII-content in the retina was approximately ten times more. Conclusion: Renin, ACE, and AngII were localized in different cell populations of the retina and there seems to be a distinct distribution pattern of corresponding AT receptors. These results support the concept of a local RAS linked to neurotransmission/neuromodulation in the retina. In addition, putative roles of the cellular RAS in growth and development have to be addressed in the retina.

Keywords: 554 retina • 488 neuropeptides • 580 signal transduction 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.