December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Impairment of Dark Adaptation in ADH4-/-RDH5-/- Double Knockout Mice
Author Affiliations & Notes
  • CM Donmoyer
    Columbia University New York NY
    Institute of Human Nutrition
  • K Lai
    Columbia University New York NY
  • J Kong
    Columbia University New York NY
  • G Duester
    Gene Regulation Program Burnham Institute La Jolla CA
  • A Molotkov
    Gene Regulation Program Burnham Institute La Jolla CA
  • WS Blaner
    Institute of Human Nutrition Medicine
    Columbia University New York NY
  • P Gouras
    Columbia University New York NY
  • Footnotes
    Commercial Relationships   C.M. Donmoyer, None; K. Lai, None; J. Kong, None; G. Duester, None; A. Molotkov, None; W.S. Blaner, None; P. Gouras, None. Grant Identification: Support: NIH Grant EY12858
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3681. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      CM Donmoyer, K Lai, J Kong, G Duester, A Molotkov, WS Blaner, P Gouras; Impairment of Dark Adaptation in ADH4-/-RDH5-/- Double Knockout Mice . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3681.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Abstract: : Purpose: To examine dark adaptation of ADH4-/-RDH5-/- double knockout mice by recording a- and b-wave amplitudes. Alcohol dehydrogenase class IV (ADH4) and cis retinol dehydrogenase type 5 (RDH5) are enzymes located in the retinal pigment epithelium. Both enzymes are able to catalyze steps needed in the visual cycle. Methods: Electroretinograms (ERGs) were performed on female age-matched, overnight dark-adapted wild type (WT; n=3), ADH4-/- (n=3), RDH5-/- (n=3), and ADH4-/-RDH5-/- (DKO; n=5) mice under anesthesia. After the dark-adapted a- and b-waves were measured, mice were light-adapted for 5 min (350 cd/m2) and the recordings were continued in the dark (flash frequency = 0.05 Hz). Body temperature was maintained using a variable heating stage. Results: Maximum b-wave amplitudes in all groups were comparable during the initial dark-adapted period, indicating the absence of retinal degeneration. In the WT, RDH5-/-, and DKO groups, b-wave amplitudes were 44%, 26% and 20% of maximum amplitudes at 5 min after the bleach, respectively. At 20 min post bleach, b-wave amplitudes were 71%, 37% and 31%. Values for ADH4-/- mice were midway between WT and DKO (32% and 43% at 5 and 20 min post bleach). There were no significant differences in the a-wave amplitudes between groups. Conclusion: The b-wave amplitudes of RDH5-/- and DKO mice were at least 50% reduced compared to WT mice at equivalent timepoints during recovery, indicating that the time course of recovery was slower in both mutant groups. ADH4-/- mice also displayed a delay in recovery relative to WT. These data suggest that these genetic knockouts give rise to an impairment of the visual cycle during dark adaptation. However, the enzymes may have redundant roles in the visual cycle. We are presently examining the biochemistry involved in these alterations.

Keywords: 571 retinoids/retinoid binding proteins • 567 retinal pigment epithelium • 396 electroretinography: non-clinical 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.