December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Transplanted Embryonic Stem Cells Differentiate and Provide Substrate for Optic Nerve Regeneration in Adult Rats
Author Affiliations & Notes
  • H Negishi
    Dept Ophthal & Vis Sci
    Grad Sch of Med Chiba Univ Chiba Japan
  • S Kubota
    Dept Ophthal & Vis Sci
    Grad Sch of Med Chiba Univ Chiba Japan
  • M Hatano
    Dept Developmental Genetics
    Grad Sch of Med Chiba Univ Chiba Japan
  • A Mizota
    Dept Ophthal & Vis Sci
    Grad Sch of Med Chiba Univ Chiba Japan
  • E Adachi-Usami
    Dept Ophthal & Vis Sci
    Grad Sch of Med Chiba Univ Chiba Japan
  • Footnotes
    Commercial Relationships   H. Negishi, None; S. Kubota, None; M. Hatano, None; A. Mizota, None; E. Adachi-Usami, None. Grant Identification: Research Fund for Community Medicine
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3696. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      H Negishi, S Kubota, M Hatano, A Mizota, E Adachi-Usami; Transplanted Embryonic Stem Cells Differentiate and Provide Substrate for Optic Nerve Regeneration in Adult Rats . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3696.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: We have reported that optic nerve fibers regenerate into an artificial graft composed of cultured Schwann cells. The purpose of this study was to determine whether transplanted embryonic stem (ES) cells can provide a substrate into which axotomized optic nerve fibers can regenerate, and whether the ES will differentiate in the artificial milieu. Methods: ES cells were suspended in an extracellular matrix, and transferred into a 1.1 cm length permeable tube (ID=1 mm) to act as a guidance channel for the artificial graft. The graft was transplanted onto the retinal stump of a transected adult rat optic nerve (Wistar strain). The relationship between the regenerating axons and the transplanted cells was analyzed by immunohistochemistry after 3 weeks. Results: The graft remained intact and was filled with parenchymatous tissue together with neovascular tissue. Immunohistochemical analysis showed that the retinal ganglion cell axons regenerated into the artificial graft and were associated with GAP-43 and cell adhesion molecules (NCAM, L1) at 3 weeks postoperation. The transplant ES cells survived and differentiated into astrocytes (GFAP positive cells), oligodendrocyte (gal-c and 04-positive cells), and neurons (tublin-positive cells) in the graft. Conclusion: These results suggest that ES cells are an efficient substrate that can support optic nerve regeneration. The ES cells can differentiate into astrocytes, oligodendrocytes, and neurons in the artificial milieu.

Keywords: 553 regeneration • 415 ganglion cells • 607 transplantation 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×