December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Endothelial Progenitor Cell-Based Delivery of TrpRS Fragments Inhibits Retinal Angiogenesis
Author Affiliations & Notes
  • A Otani
    Department of Cell Biology
    The Scripps Research Institute La Jolla CA
  • K Kinder
    Department of Cell Biology
    The Scripps Research Institute La Jolla CA
  • K Ewalt
    Department of Chemical and Molecular Biology
    The Scripps Research Institute La Jolla CA
  • B Slike
    Department of Chemical and Molecular Biology
    The Scripps Research Institute La Jolla CA
  • F Otero
    Department of Chemical and Molecular Biology
    The Scripps Research Institute La Jolla CA
  • P Schimmel
    Department of Chemical and Molecular Biology
    The Scripps Research Institute La Jolla CA
  • M Friedlander
    Department of Cell Biology
    The Scripps Research Institute La Jolla CA
  • Footnotes
    Commercial Relationships   A. Otani, None; K. Kinder, None; K. Ewalt, None; B. Slike, None; F. Otero, None; P. Schimmel, None; M. Friedlander, Merck KGaA F. Grant Identification: NEI P30 EY12598-01; 5R01 EY12599-03 MF, Robert Mealey Center for the Study of Macular Degeneration
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3715. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      A Otani, K Kinder, K Ewalt, B Slike, F Otero, P Schimmel, M Friedlander; Endothelial Progenitor Cell-Based Delivery of TrpRS Fragments Inhibits Retinal Angiogenesis . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3715.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: We have recently reported that fragments of tryptophanyl-tRNA synthetase (T2) are potent anti-angiogenics. We have also shown that endothelial progenitor cells localize to, and become part of, newly forming murine retinal vasculature. This study utilizes cell-based delivery of T2 to inhibit angiogenesis in a murine retinal model. Methods: MACS was used to separate Lin- progenitor cells from murine bone marrow. Cells were transfected with a plasmid encoding an IgΚ chain signal sequence and T2. Transfected cells were injected intravitreally into postnatal day 3 murine eyes. Eyes were harvested nine days later and were stained with Collagen IV antibody to analyze the effect on the developing vascular network. Results: Western blot analysis confirmed the production of secreted T2 by transfected endothelial progenitor cells. Eyes receiving T2 transfected endothelial progenitor cells exhibited complete disruption of ongoing retinal angiogenesis. Injection of cells transfected with control plasmid, had no effect on angiogenesis. Conclusion: These results demonstrate that cell-based delivery can be used to selectively target neovascularization in the eye with novel, potent anti-angiogenics.

Keywords: 483 neovascularization • 566 retinal neovascularization 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×