December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Inhibition of Capillary Morphogenesis In Vitro by Ex Vivo Expanded Limbal Epithelial Cells Cultivated on Preserved Human Amniotic Membrane
D Ma; HJ Wu; RJ F Tsai; JK Chen; LK Yeh
Author Affiliations & Notes
  • D Ma
    Ophthalmology/Chang-Gung Hosp 6F-1 #16 Alley 14 Lane 283 Taipei Taiwan Republic of China
  • HJ Wu
    Department of Ophthalmology Chang Gung Memorial Hospital Linko Taiwan Republic of China
  • RJ F Tsai
    Department of Ophthalmology Chang Gung Memorial Hospital Linko Taiwan Republic of China
  • JK Chen
    Department of Physiology Chang Gung University Linko Taiwan Republic of China
  • LK Yeh
    Department of Ophthalmology Chang Gung Memorial Hospital Linko Taiwan Republic of China
  • Footnotes
    Commercial Relationships   D. Ma, None; H.J. Wu, None; R.J.F. Tsai, None; J.K. Chen, None; L.K. Yeh, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3718. doi:
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      D Ma, HJ Wu, RJ F Tsai, JK Chen, LK Yeh; Inhibition of Capillary Morphogenesis In Vitro by Ex Vivo Expanded Limbal Epithelial Cells Cultivated on Preserved Human Amniotic Membrane . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3718.

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Abstract

Abstract: : Purpose: To understand the anti-angiogenic effect of ex vivo expanded human limbal epithelial cells (HLE) cultivated on preserved human amniotic membrane (HAM). Methods: Human umbilical vein endothelial cells (HUVEC) were seeded in type I collagen gel in a transwell, which was in turn cocultured with human limbal fibroblasts (HLF) seeded in three-dimensional type I collagen gel containing (1) no HAM (2) HAM only (3) HLE cultivated on HAM (HLE/HAM). After three days, total capillary tube length was measured and analyzed by digital image analyzer. Results: HLF in type I collagen gel induced prominent capillary tube formation by HUVEC. Capillary tube length in the HLF gel containing only HAM group was only mildly decreased. However, capillary tube length in the HLF gel containing HLE/HAM group was significantly reduced. Conclusion: HAM alone express anti-angiogenic activity in vitro, and this effect may be further augmented by HLE, which may be responsible for the clinical anti-angiogenic/anti-inflammatory effect of HLE/HAM transplantation.

Keywords: 372 cornea: epithelium • 437 inflammation • 607 transplantation 
© 2002, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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