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YS Yu, J Kim, J Kim, J Seo, S-J Kim, H Yu, H Chung; Protective Effect of Heat Shock Protein (hsp)70.1 and hsp70.3 on Retinal Photic Injury After Systemic Hyperthermia . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3727.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To determine the relation between hsp70’s from hsp70.1 and 70.3 on retinal photic injury after systemic hyperthermia Methods: 5-week-old female C57BL/6 mice were dipped in a darkened water bath kept at a constantly monitored temperature of 41-42°C for 25-30 minutes. After dark adaptation for 8 hours, the intense diffuse, white, cool fluorescent light was maintained with the equidirectional intensity of 11000 lux for 6 hours. At 1 day, 4 days, 7 days and 14 days after the light stress, mice were sacrificed. Histology, immunohistochemistry for constitutive hsp70 and inducible hsp70, western blot analysis, RT-PCR for hsp70.1 and 70.3 were performed. Results: There were no definite changes on histologic examination after the light stress with hyperthermia. Immunoreactivity of inducible hsp70 was densely expressed in inner segment of photoreceptor layer, and that of constitutive hsp70 was prominent at the same site, though faint. Production of hsp70 reached peak at 1 day, and slowly decreased. mRNA from hsp70.3 was induced earlier than that of hsp70.1. Conclusion: As previously known, hsp70 had a protective effect on retinal photic injury. Newly, we found hsp70 from hsp70.3 may be a rapid responder, and hsp70.1 a slower responder. That is, hsp70 from hsp70.3 may be a major initial protector on retinal photic injury.
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