December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
The Effects of Nitric Oxide on GABA Receptor Function in the Rod Bipolar Cells of the Rat Retina
Author Affiliations & Notes
  • C-S Jung
    Department of Biophysics
    College of Medicine The Catholic University of Korea Seoul 137-701 Republic of Korea
  • NG Park
    Department of Biophysics
    College of Medicine The Catholic University of Korea Seoul 137-701 Republic of Korea
  • SS Paik
    Department of Biophysics
    College of Medicine The Catholic University of Korea Seoul 137-701 Republic of Korea
  • EJ Lee
    Department of Anatomy
    College of Medicine The Catholic University of Korea Seoul 137-701 Republic of Korea
  • SJ Lee
    Department of Biophysics
    College of Medicine The Catholic University of Korea Seoul 137-701 Republic of Korea
  • SH Bai
    Department of Biophysics
    College of Medicine The Catholic University of Korea Seoul 137-701 Republic of Korea
  • MH Chun
    Department of Anatomy
    College of Medicine The Catholic University of Korea Seoul 137-701 Republic of Korea
  • Footnotes
    Commercial Relationships   C. Jung, None; N.G. Park, None; S.S. Paik, None; E.J. Lee, None; S.J. Lee, None; S.H. Bai, None; M.H. Chun, None. Grant Identification: Support: The Korea Science and Engineering Foundation (2000-1-20700-008-3).
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3773. doi:
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    • Get Citation

      C-S Jung, NG Park, SS Paik, EJ Lee, SJ Lee, SH Bai, MH Chun; The Effects of Nitric Oxide on GABA Receptor Function in the Rod Bipolar Cells of the Rat Retina . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3773.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To investigate the effects of nitric oxide on the GABA receptor function in the rod bipolar cells of the rat retina. Methods: GABA induced currents were recorded by the whole cell patch clamp method in the rod bipolar cells enzymatically dissociated from retina. After recording, bipolar cells were processed for PKC immunoreactivity. Results: Rod bipolar cells were identified by their morphology and positive immunoreactivity to an antiserum against PKC. GABA (100µM)-activated currents on the rod bipolar cells comprised a fast and sustained part. The fast and the sustained GABA responses were blocked by bicuculline (200 µM; an antagonist of GABAA receptor) and imidazole-4-acetic acid (200 µM; an antagonist of GABAC rceptor), respectively. The GABA responses were completely blocked by picrotoxin (1 mM; a Cl¯ channel blocker). Muscimol (50 µM; an agonist at GABAA receptor) and trans-4-aminocrotonic acid (300 µM; an agonist at GABAC receptor) elicited the current, but baclofen (1 mM; a GABAB receptor agonist) did not minic the GABA effect. Sodium nitroprusside (1mM; a NO donor) reduced the fast part of GABA currents in the rod bipolar cells. The fast responses evoked by muscimol (50 µM) were also reduced in the presence of the NO donor. Conclusion: These results show that NO decrease GABAA receptor function of the rod bipolar cells in the rat retina.

Keywords: 330 bipolar cells • 491 nitric oxide • 541 receptors: pharmacology/physiology 
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