December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
The In Vitro Evaluation of the New Antimicrobial Agent N-Chlorotaurine (NCT) Against Ocular Isolates of Adenovirus
EG Romanowski; KA Yates; B Teuchner; M Nagl; W Gottardi; YJ Gordon
Author Affiliations & Notes
  • EG Romanowski
    The Charles T Campbell Laboratory University of Pittsburgh Pittsburgh PA
  • KA Yates
    The Charles T Campbell Laboratory University of Pittsburgh Pittsburgh PA
  • B Teuchner
    University of Innsbruck Innsbruck Austria
  • M Nagl
    University of Innsbruck Innsbruck Austria
  • W Gottardi
    University of Innsbruck Innsbruck Austria
  • YJ Gordon
    The Charles T Campbell Laboratory University of Pittsburgh Pittsburgh PA
  • Footnotes
    Commercial Relationships   E.G. Romanowski, None; K.A. Yates, None; B. Teuchner, None; M. Nagl, None; W. Gottardi, None; Y.J. Gordon, None. Grant Identification: NIH Grant EY08227 and NIH Core Grant EY08098
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3858. doi:
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      EG Romanowski, KA Yates, B Teuchner, M Nagl, W Gottardi, YJ Gordon; The In Vitro Evaluation of the New Antimicrobial Agent N-Chlorotaurine (NCT) Against Ocular Isolates of Adenovirus . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3858.

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Abstract

Abstract: : Purpose: To evaluate the in vitro efficacy of N-chlorotaurine (NCT), an endogenous microbicidal oxidant produced by stimulated human leukocytes, against multiple ocular isolates of adenovirus (Ad). Methods: In duplicate trials, the direct inactivating activity of NCT was determined by incubating high titer stocks of clinical isolates of Ad1, Ad2, Ad3, Ad4, Ad5, Ad7a, Ad8, Ad19, and Ad37 ATCC with 2.5%, 1.0%, 0.5%, and 0.1% NCT and control media for 60 minutes at room temp. Standard plaque assays were performed on the reaction mixtures to determine the Ad titers after NCT or control treatment. Ad titers were Log converted and expressed as the mean sd pfu/ml. Results: Mean Ad Titer (Log10 pfu/ml)  

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Conclusions: NCT demonstrated concentration-dependent direct inactivation of all ocular Ad isolates tested. NCT concentrations of 0.5% or greater demonstrated at least a 3-Log reduction in the mean titers of all Ad isolates. Further study of NCT as an antiviral agent against adenovirus is warranted.

Keywords: 307 adenovirus • 322 antiviral drugs • 366 conjunctivitis 
© 2002, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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