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AS Wilson, B Hobbs, W Shen, T Speed, G Begley, P Rakoczy; Microarray Analysis of Retinal Laser Photocoagulation . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3926.
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Purpose: Laser photocoagulation has proven to be successful in the treatment of a variety of ocular disorders. However, the underlying mechanisms that occur as a result of this treatment have yet to be elucidated. The current study utilises microarray technology to examine the short-term altered mRNA expression in retinal tissue following laser treatment. Methods: Argon laser irradiation was delivered into the left eye of normal C57Bl/6J mice (n = 10), with the right eye serving as control for each animal. The mice were euthanased and enucleated 3d-post laser treatment. The retinas from control and treated eyes were dissected and pooled into respective groups and the total RNA extracted and purified. Biotinylated cRNA was then generated, purified and fragmented prior to hybridization to the MU74Av2 Affymetrix GeneChip. The MU74Av2 GeneChip contains ∼6,000 functionally characterized genes and ∼6,000 EST clusters. Replicate hybridizations using separate chips were performed for both control and laser treated samples. Data comparisons between control and treated samples were performed and statistically analyzed. Results: Data reveal that laser photocoagulation modulated gene expression of several classes of genes including, adhesion molecules, growth factors, transcription factors, intracellular signalling molecules, and factors involved in wound healing and repair, inflammation and angiogenesis. Quantitative analysis using real time PCR was adopted to confirm the changes in expression of specific genes of interest. Conclusion: Microarray technology has proven a useful tool in examining the effects of laser photocoagulation on retina and further analysis of the data may reveal the process by which laser therapy is effective as a treatment of ocular disorders. Acknowledgments: The authors acknowledge with thanks the financial support provided by the National Health and Medical Research Council (Australia), the Juvenile Diabetes Research Foundation (USA) and WestPac Australia. This work is part of the research effort of the Diabetic Retinopathy Consortium, Perth.
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