December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Microarray Analysis of Retinal Laser Photocoagulation
AS Wilson; B Hobbs; W Shen; T Speed; G Begley; P Rakoczy
Author Affiliations & Notes
  • AS Wilson
    Molecular Ophthalmology Lions Eye Institute Nedlands WA Australia
  • B Hobbs
    Division of Genetics and Bioinformatics Walter and Eliza Hall Institute for Medical Research Melbourne VIC Australia
  • W Shen
    Centre for Ophthalmology and Visual Science University of Western Australia Perth WA Australia
  • T Speed
    Division of Genetics and Bioinformatics Walter and Eliza Hall Institute for Medical Research Melbourne VIC Australia
  • G Begley
    Institute for Child Health Research Perth WA Australia
  • P Rakoczy
    Centre for Ophthalmology and Visual Science University of Western Australia Perth WA Australia
  • Footnotes
    Commercial Relationships   A.S. Wilson, None; B. Hobbs, None; W. Shen, None; T. Speed, None; G. Begley, None; P. Rakoczy, None. Grant Identification: Support: NH&MRC grant, JDRF program grant
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 3926. doi:
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      AS Wilson, B Hobbs, W Shen, T Speed, G Begley, P Rakoczy; Microarray Analysis of Retinal Laser Photocoagulation . Invest. Ophthalmol. Vis. Sci. 2002;43(13):3926.

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Abstract

Abstract: : Purpose: Laser photocoagulation has proven to be successful in the treatment of a variety of ocular disorders. However, the underlying mechanisms that occur as a result of this treatment have yet to be elucidated. The current study utilises microarray technology to examine the short-term altered mRNA expression in retinal tissue following laser treatment. Methods: Argon laser irradiation was delivered into the left eye of normal C57Bl/6J mice (n = 10), with the right eye serving as control for each animal. The mice were euthanased and enucleated 3d-post laser treatment. The retinas from control and treated eyes were dissected and pooled into respective groups and the total RNA extracted and purified. Biotinylated cRNA was then generated, purified and fragmented prior to hybridization to the MU74Av2 Affymetrix GeneChip. The MU74Av2 GeneChip contains ∼6,000 functionally characterized genes and ∼6,000 EST clusters. Replicate hybridizations using separate chips were performed for both control and laser treated samples. Data comparisons between control and treated samples were performed and statistically analyzed. Results: Data reveal that laser photocoagulation modulated gene expression of several classes of genes including, adhesion molecules, growth factors, transcription factors, intracellular signalling molecules, and factors involved in wound healing and repair, inflammation and angiogenesis. Quantitative analysis using real time PCR was adopted to confirm the changes in expression of specific genes of interest. Conclusion: Microarray technology has proven a useful tool in examining the effects of laser photocoagulation on retina and further analysis of the data may reveal the process by which laser therapy is effective as a treatment of ocular disorders. Acknowledgments: The authors acknowledge with thanks the financial support provided by the National Health and Medical Research Council (Australia), the Juvenile Diabetes Research Foundation (USA) and WestPac Australia. This work is part of the research effort of the Diabetic Retinopathy Consortium, Perth.

Keywords: 554 retina • 454 laser • 417 gene/expression 
© 2002, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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