Purchase this article with an account.
Z-H Song, J Jiang, A Hemesath, C McCloud, L Zhong; Anterior Segment CB1 and CB2 Cannabinoid Receptors . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4027.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: To examine the expression of CB1 and CB2 cannabinoid receptors in the anterior segment of the eye. Methods: Northern and in situ hybridization histochemistry were used to examine the CB2 mRNA expression in the tissue sections of the eye, and the technique of immunofluorescent microscopy was used to localize cannabinoid receptor proteins. In addition, cAMP accumulation and MAP kinase assays were used to identify functional cannabinoid receptors in cultured ocular cells. Results: Using the specific anti-sense probe to CB2 mRNA, positive hybridization signals were detected in cornea, iris, ciliary body, and trabecular meshwork. In contrast, using the control sense probe, no hybridization signal was detected in any of the above regions. With immunofluorescent microscopy, positive signals were detected on ciliary epithelial and trabecular meshwork cells with anti-CB1 and anti-CB2 antibodies. In contrast, no positive signals were detected when the primary antibodies were preabsorbed with peptide antigen. In functional assays, cannabinoid agonists inhibited forskolin-stimulated cAMP accumulation and stimulated p42/44 MAP kinase activity in ciliary epithelial and trabecular meshwork cells. Conclusion: These data demonstrate that the mRNA and protein for both CB1 and CB2 cannabinoid receptors are located in the anterior chamber of the eye. These results also indicate that functionally active cannabinoid receptors are present in those anterior segment cells that are important for maintaining IOP.
This PDF is available to Subscribers Only