December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Experimental Model for Optic Nerve Protection Studies
Author Affiliations & Notes
  • N Callizot
    Neurofit Illkirch France
  • A-C Fintz
    Neurofit Strasbourg France
  • CL Percicot
    Novartis Ophtalmics Strasbourg France
  • M Simonutti
    Laboratoire de Physiopathologie Moléculaire et Cellulaire de la Rétine Strasbourg France
  • J Sahel
    Laboratoire de Physiopathologie Moléculaire et Cellulaire de la Rétine Strasbourg France
  • GN Lambrou
    Novartis Ophtalmics Strasbourg France
  • Footnotes
    Commercial Relationships   N. Callizot, None; A. Fintz, None; C.L. Percicot, None; M. Simonutti, None; J. Sahel, None; G.N. Lambrou, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 4049. doi:
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      N Callizot, A-C Fintz, CL Percicot, M Simonutti, J Sahel, GN Lambrou; Experimental Model for Optic Nerve Protection Studies . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4049.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose: To develop a model of optic nerve degeneration in adult mouse mice and assess the effect of the lesion with electrophysiology and histology. Methods: Unilateral optic nerve crushes were performed with a self-closing gentle tensioned forceps in 10 weeks-old C57Bl/6J male mice. Control group were sham-operated. Binocular and monocular recordings of visually evoked potentials (VEP) signals were performed. Latency and amplitude of VEP were studied. Number of optic nerve fibers were estimated 4 weeks after the crush in both group. Results: No significant differences were observed in the bilateral recordings of VEP. Latencies slightly increased and amplitudes of the signal were significantly weaker in crushed compared to sham-operated eyes from 10 days after the crush in monocular recordings. Numbers of myelinized fibers were significantly reduced in crushed nerve as in the centre as in the periphery of the nerve. Conclusion: The results with VEP reflected a visual impairment which is confirmed by histological analysis of the optic nerve fibers number. This experimental nerve degeneration is suitable for in vivo studies of optic nerve protection especially in the case of glaucoma.

Keywords: 385 degenerations/dystrophies • 506 pathology: experimental • 489 neuroprotection 

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