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T Tungsiripat, MA Sarayba, L Li, PM Sweet, RS Chuck; A Portable Anterior Corneal Harvesting Device . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4165.
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Purpose: To determine the reproducibility of anterior sclerokeratectomy using a portable non-electric microkeratome-based device for harvesting the entire anterior corneal surface for lamellar transplantation. Methods: A portable non-electric microkeratome-based device was composed of a simple manual vacuum pump (MityVac, San Antonio,TX) and a modified gas turbine-driven microkeratome (LSK One, Moria/Microtech, Doylestown, PA) attached to a 16 mm suction ring. This system, capable of removing the entire corneal surface including the limbus and adjacent sclera, was evaluated. Porcine globes (n=25) were randomly separated into two groups in which different depth heads (170 vs. 200 µm) were used under pressure created by the hand pump (58-64 cmHg). The cut reproducibility was assessed by measuring the harvested disc diameter using planimetry after digital photography and measuring the thickness at five points by ultrasound pachymetry. Results: The manual vacuum pump was able to achieve an average suction pressure of 63.0 ± 1.6 cmHg. Loss of suction during the pass was observed in 4 cases using the 170 µm (31%) head and in 1 case using the 200 µm head (8.3%). A central buttonhole was observed in 1 case for each head size (7.7% for the 170 µm head and 8.3% for the 200 µm head). The obtained discs were fairly circular (horizontal vs. vertical diameters, P ≷ 0.2), with average diameters of 12.85 ± 0.52 mm and 13.25 ± 1.15 mm for the 170 and 200 µm heads, respectively. The average central disc thickness obtained was 176.92 ± 34.68 µm and 166.00 ± 53.74 µm for the 170 and 200 µm heads, respectively. Conclusions: This portable anterior corneal harvesting instrument is a promising device to obtain corneal discs of reproducible diameter and thickness and presents an economical and portable alternative which could be used by eye banks worldwide. CR: None. Support: LASIK Institute and Moria/Microtech Inc. Sclerocorneal Lenticule Dimensions,Mean(SD),[Range] View OriginalDownload SlideView OriginalDownload Slide
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