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N Hashizume, S Saika, Y Okada, Y Ohnishi; The Roles Of Hyaluronic Acid Synthesis In Corneal Epithelial And Endothelial Spreading . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4203.
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Purpose: We examined the roles of hyaluronic acid synthesis in corneal epithelial or endothelial cells in organ culture. Methods: The effects of hyaluronic acid and of inhibition of hyaluronic acid by adding 4-methylumbelliferone into culture medium on spreading of rabbit corneal epithelium or endothelial cells were examined in organ culture. An endothelial defect was produced by scraping in corneal brocks(2x2mm) obtained from adult albino rabbit., then the corneal brocks were organ-cultured for 24hrs with or without the agents. Endothelial healing was investigated by Arizalinin red. Corneal epithelial path was also assayed in this system. Results: Organ culture experiments revealed hyaluronic acid promoted the corneal epithelium and endothelial cells. Inhibition of hyaluronic acid synthesis by 4-methylumbelliferone suppressed their spreading. Adding hyaluronic acid partially recovered the effects of 4-methylumbelliferone. Conclusion: Endogenous hyaluronic acid synthesis is required for movement of corneal epithelium and endothelial cells.
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