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Y Okada, S Saika, K Shirai, E Senba, Y Ohnishi; AP-1(c-fos/c-jun) is Required for Corneal Epithelial Movement . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4209.
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Purpose:We have demonstrated that AP-1 compoments, c-fos/c-jun, are up-regulated in rat migrating corneal epithelium in relatively early phase of healing, suggesting the AP-1 function of initiation of cell movement. To explore this hypothesis, we examined the effect of lacking c-Fos and c-Jun protein expressions on the spreading of corneal epithelium in situ in organ-culture. Antisense-oligonucleotide (AS) was used for this purpose. Methods:Central corneas of C57BL/6 mice were obtained from each eye. These corneal blocks (2x2mm) were incubated for 11h in Eagle`s minimum essential medium with 8 µM c-fos AS or c-jun AS probe. Sense probe (S) was used for negative control. Cryosections were stained with hematoxylin-eosin and photographed under light microscopy. The length of the path of epithelial spreading on stromal cut surface (both sides) was measured. Data were analyzed by unpaired Student's t-test. Results:Adding c-fos AS to the medium decreased the length of epithelial spreading rate to 40.36% of control with the S probe. Addition of c-jun AS decreased the length of epithelial spreading rate to 42.71% of control with S probe. Conclusion:AP-1 (c-Fos/c-Jun) is essential molecules for the corneal epithelial spreading.
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