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J Cluzel, A Abbas, B Plazonnet, J-M Aiache, M Doly; Investigation of Ocular Healing and Anti-Inflammatory Activities of Hibiscus Sabdariffa in Rabbits . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4220.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Ocular aqueous preparation of Hibiscus sabdariffa (HS) was evaluated for ocular healing and inflammation following sodium hydroxide burning and intraocular injection of lipoloysaccharide (LPS). Methods: The flowers and calyxes of HS plant were coarsly powdered and extracted using de-ionised water as the solvent to prepare 0.1 % w/v. The water extracts obtained were neutralised to pH 7 and diluted using isotonic phosphate buffer to different concentration ranges (0.1 to 0.5 %). The Intra-ocular inflammation was induced by intravitreal injection of 12.5 µg LPS from E. Coli. Corneal burning was made by application of 1 N sodium hydroxide for 20 s. Histologic and Ophthalmic examinations were performed 2 and 7 days after injury, and aqueous protein concentrations and cell counts were also determined. Commercial Indomethacine was used as a reference drug. Results: Intra-ocular inflammation appeared within 12 hrs of LPS injection, became maximal at 48 hrs post-injection, and lasted for nearly 7 days. The percent reduction in leukocyte count was 80,5 % with 0.1 % HS preparation and about 87.2 % with indomethacine eye drops. At post-burn day 2, oedema, haze and neovascularization have occured in the control group whereas weaker scoring was observed in the treated-group. Conclusion: This preliminary study demonstrate that ocular aqueous preparation of HS could play a preventive role against inflammation and ulceration through anti-oxidative mechanisms or via other mechanisms. This extract is highly enriched in polyphenol, protocatechins and anthocyanidins but additional studies are under investigation to analyse more precisely the composition of the extract.
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