December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Expression of Type XVIII Collagen During Wound Healing of Corneal Incisions and Keratectomy Wounds
T Kato; J-H Chang; DT Azar
Author Affiliations & Notes
  • T Kato
    Schepens Eye Research Institute and Massachusetts Eye & Ear Infirmary Harvard Medical School Boston MA
  • J-H Chang
    Schepens Eye Research Institute and Massachusetts Eye & Ear Infirmary Harvard Medical School Boston MA
  • DT Azar
    Schepens Eye Research Institute and Massachusetts Eye & Ear Infirmary Harvard Medical School Boston MA
  • Footnotes
    Commercial Relationships   T. Kato, None; J. Chang, None; D.T. Azar, None. Grant Identification: NIH Grant NEI 10101
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 4231. doi:
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      T Kato, J-H Chang, DT Azar; Expression of Type XVIII Collagen During Wound Healing of Corneal Incisions and Keratectomy Wounds . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4231.

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Abstract

Abstract: : Purpose:: To determine the distribution of type XVIII collagen in mouse ocular tissues and to investigate the expression of type XVIII collagen during corneal wound healing of corneal incisions and keratectomy wounds. Methods:We performed immunohistochemical analysis of type XVIII collagen in mouse ocular tissue using polyclonal antibodies to the hinge domain. For wound healing experiment, we performed excimer laser keratectomy and single linear incisions on mouse corneas. The corneas were harvested at various times after wounding and processed for either immunohistochemistry, in situ hybridization, competitive polymerase chain reaction (PCR) or western blotting. Results:In the mouse ocular tissues, type XVIII collagen was immunolocalized to the corneal epithelium, ciliary epithelium, lens capsule, retinal internal limiting membrane, and Bruch’s membrane. After excimer laser keratectomy, type XVIII collagen was expressed in the corneal epithelium. After lineal corneal incisions type XVIII collagen was also expressed in the corneal epithelium. In addition, type XVIII collagen was immunolocalized to the subepithelial stromal wound healing region 1 week after wounding and its mRNA showed corresponding temporal increased expression in the same region. Conclusion:Our results suggest that type XVIII collagen is broadly expressed in ocular tissues and that it may have a potential role in wound healing after incisional corneal wounds.

Keywords: 370 cornea: basic science • 403 extracellular matrix • 631 wound healing 
© 2002, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
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