Purchase this article with an account.
T Miyamoto, S Saika, Y Okada, A Yamanaka, Y Suzuki, Y Ohnishi; Comparison of Epithelial and Stromal Wound Healing Between Following PRK and LASIK in Rabbits: Expression of COX2, Alpha Smooth Muscle Actin and HSP47 . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4234.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose:To compare the epithelial and stromal wound healing processes of rabbit corneas treated with either photorefrative keratectomy (PRK) or laser in situ keratomileusis (LASIK) for same refractive correction. Methods:Right eye of adult albino rabbits (n=24) were treated with PRK or LASIK for 5 diopter correction. After intervals of healing for 3 days, 4 weeks, 3 and 6 months, the animals were killed and each cornea was histologically and immunohistochemically examined for α-smooth muscle actin (αSMA), heat shock protein 47 (HSP47) and cyclooxigenase 2 (COX2). Results:Keratocytes in the central anterior stroma were positive for HSP47 at Day 3, and for αSMA at Week 4 in PRK-treated cornea, whereas they were negative throughout the healing interval in LASIK-treated corneas. Whole corneal epithelium was found to express COX2 protein at Day 3 following PRK. In LASIK-treated cornea, it was positive in the epithelium adjacent to the edge of the flap and also the central epithelium. At Week 4, COX2 was detected in the central corneal epithelium of PRK-treated cornea, whereas it was not seen there in LASIK-treated cornea. After 3 months, entire epithelium was negative in both groups. Conclusion:Expression of HSP47 and αSMA in keratocytes in PRK-treated corneas may indicate the severity of stromal damage. Coverage of stromal wound by the flap might reduce the keratocyte response. Spatial and temporal expression patterns of COX2 were different between PRK- and LASIK-treated rabbit corneas. LASIK procedure might stimulate the central intact epithelium of the flap to express COX2.
This PDF is available to Subscribers Only