December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Expression of CC Chemokines During Immune Clearance of HSV-1 from the Infected Cornea
Author Affiliations & Notes
  • RN Lausch
    University of South Alabama Mobile AL
  • AD Coleman
    University of South Alabama Mobile AL
  • SJ Molesworth-Kenyon
    University of South Alabama Mobile AL
  • JE Oakes
    University of South Alabama Mobile AL
  • Footnotes
    Commercial Relationships   R.N. Lausch, None; A.D. Coleman, None; S.J. Molesworth-Kenyon, None; J.E. Oakes, None. Grant Identification: NIH EY07564
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 4305. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      RN Lausch, AD Coleman, SJ Molesworth-Kenyon, JE Oakes; Expression of CC Chemokines During Immune Clearance of HSV-1 from the Infected Cornea . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4305.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Abstract: : Purpose: The CC chemokines MIP-1α, MIP-1ß, and MCP-1 are expressed in virus-infected tissues. We investigated whether these chemokines played a role in immune clearance of HSV-1 from the infected eye. Methods: C57Bl/6 wildtype (WT) and MIP-1α-/- knockout (KO) mice immunized subcutaneously to HSV-1 were challenged 10 days later topically on the scarified cornea with 2x104 PFU HSV-1 strain RE. Eyes were collected 48 hrs later and tissue lysates were assayed for infectious virus by plaque assay and chemokines by ELISA. Results: Two days after virus challenge HSV-1 titers in the eyes of immune WT hosts were consistently 10 to 100-fold lower than that observed in the eyes of immunized KO hosts. Depletion of CD4+ T cells but not CD8+ T cells abrogated immune clearance. Substantial levels of MIP-1α (≷100pg/ml) were detected in immunized WT corneas whereas no MIP-1α was detected in KO hosts, or naïve WT mice. MIP-1ß was some 18-fold higher in immune WT than in naïve WT host and 4-fold higher in immune WT compared to immune KO mice. In contrast, MCP-1 levels were only 2-fold higher versus naïve WT, and not significantly higher than that found in immune KO mice. Conclusion: Our results suggest that MIP-1α is required for efficient HSV-1 clearance from the cornea. MIP-1ß may also be needed whereas MCP-1 appears to be of lesser importance.

Keywords: 380 cytokines/chemokines • 425 herpes simplex virus • 437 inflammation 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.