December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Effect of Immunosuppression on Gene Expression in the HSV-1 Latently Infected Mouse Trigeminal Ganglion
Author Affiliations & Notes
  • S Higaki
    Ophthalmology LSU Eye Center New Orleans LA
  • BM Gebhardt
    Ophthalmology LSU Eye Center New Orleans LA
  • WJ Lukiw
    LSU Neuroscience Center New Orleans LA
  • HW Thompson
    Ophthalmology LSU Eye Center New Orleans LA
  • Y Shimomura
    Ophthalmology Kinki University School of Medicine Osaka-Sayama Japan
  • JM Hill
    Ophthalmology LSU Eye Center New Orleans LA
  • Footnotes
    Commercial Relationships   S. Higaki, None; B.M. Gebhardt, None; W.J. Lukiw, None; H.W. Thompson, None; Y. Shimomura, None; J.M. Hill, None. Grant Identification: R01EY06311, EY02377, R01AG18031, Senior Scientific
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 4310. doi:
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    • Get Citation

      S Higaki, BM Gebhardt, WJ Lukiw, HW Thompson, Y Shimomura, JM Hill; Effect of Immunosuppression on Gene Expression in the HSV-1 Latently Infected Mouse Trigeminal Ganglion . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4310.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose:To determine changes in gene expression in HSV-1 latently infected mouse trigeminal ganglia (TGs) following treatment with cyclophosphamide and dexamethasone. Methods: Scarified corneas of BALB/c mice were inoculated with 2.5 X 104 PFU HSV-1 strain McKrae. Four weeks after inoculation, cyclophosphamide and dexamethasone were intravenously injected to induce HSV-1 reactivation. Uninfected mice were also treated with the drugs. Four groups of mice were studied: 1) uninfected, not treated, 2) uninfected, drug treated, 3) latently infected, not treated, 4) latently infected, drug treated. Poly A+ mRNA from the TGs of each group was reverse transcribed, labeled with 32P, incubated on a 1185-gene array membrane, and analyzed by phosphorimaging. To confirm microarray results, semi-quantitative RT-PCR for selected genes was performed. Results: A statistically significant increase in the expression of 10 genes (prostaglandin E2 receptor EP4 subtype, insulin promoter factor 1, glutathione S-transferase mu 2, cyclin D2, peripherin, plasma glutathione peroxidase, methyl CpG-binding protein 2, retinal S-antigen, ErbB2 proto-oncogene, guanine nucleotide-binding protein alpha stimulating activity polypeptide) and decreased transcriptional activity of 8 genes (peripheral myelin protein 22, decorin, transcription factor AP-1, dystroglycan 1, myelin protein 0, mitogen-activated protein kinase 3, prothymosin beta 4, brain lipid-binding protein) were documented in the ganglia of latently infected immunosuppressed mice. The results obtained by semi-quantitative RT-PCR were similar to those obtained by microarray analysis. Conclusion: Changes in gene expression in the prostaglandin pathway, transcription factors, and enzymes in the cell cycle may be important in HSV-1 reactivation induced by immunosuppression.

Keywords: 425 herpes simplex virus • 417 gene/expression 

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