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M Paques, A Regner, O Genevois, R Sercombe, R Tadayoni, E Vicaut, A Gaudric; Indocyanine Green is a Fast Bidirectional Axonal Tracer . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4347.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:To analyze the axon-tracing properties of indocyanine green (ICG). Methods:ICG was instilled in the vitreous or in the superior colliculus of rabbits and rats. Infrared fluorescence in the retina and the brain was subsequently revealed using a standard fundus camera with ICG filters. Results:After being introduced into the vitreous, ICG fluorescence was observed along the visual pathway up to the geniculate body and colliculus without detectable extracellular leakage. The fluorescence persisted at least 2 months. After instillation into the superior colliculus, retinal ganglion cell fluorescence was observed in the fundus of the awake rat. The axonal speed of ICG was estimated between 2 and 4 mm/hour. Conclusion:ICG is a convenient bidirectional fast axonal tracer, allowing to observe in the awake rat retrogradely labelled retinal ganglion cells with a standard fundus ICG camera.
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