December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Indocyanine Green is a Fast Bidirectional Axonal Tracer
Author Affiliations & Notes
  • M Paques
    Opthalmology
    Hopital Lariboisiere Paris France
  • A Regner
    CNRS U 646 Paris France
  • O Genevois
    Laboratoire d'etude de la Microcirculation Paris France
  • R Sercombe
    CNRS U 646 Paris France
  • R Tadayoni
    Ophthalmology
    Hopital Lariboisiere Paris France
  • E Vicaut
    Laboratoire d'etude de la Microcirculation Paris France
  • A Gaudric
    Ophthalmology
    Hopital Lariboisiere Paris France
  • Footnotes
    Commercial Relationships   M. Paques, None; A. Regner, None; O. Genevois, None; R. Sercombe, None; R. Tadayoni, None; E. Vicaut, None; A. Gaudric, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 4347. doi:https://doi.org/
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    • Get Citation

      M Paques, A Regner, O Genevois, R Sercombe, R Tadayoni, E Vicaut, A Gaudric; Indocyanine Green is a Fast Bidirectional Axonal Tracer . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4347. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose:To analyze the axon-tracing properties of indocyanine green (ICG). Methods:ICG was instilled in the vitreous or in the superior colliculus of rabbits and rats. Infrared fluorescence in the retina and the brain was subsequently revealed using a standard fundus camera with ICG filters. Results:After being introduced into the vitreous, ICG fluorescence was observed along the visual pathway up to the geniculate body and colliculus without detectable extracellular leakage. The fluorescence persisted at least 2 months. After instillation into the superior colliculus, retinal ganglion cell fluorescence was observed in the fundus of the awake rat. The axonal speed of ICG was estimated between 2 and 4 mm/hour. Conclusion:ICG is a convenient bidirectional fast axonal tracer, allowing to observe in the awake rat retrogradely labelled retinal ganglion cells with a standard fundus ICG camera.

Keywords: 474 microscopy: light/fluorescence/immunohistochemistry • 557 retina: proximal(bipolar, amacrine, and ganglion cells) • 593 superior colliculus/optic tectum 
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