December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Novel Nanoparticles For Contrast Enhancement Optical Coherence Tomography In Doppler
Author Affiliations & Notes
  • MR Glucksberg
    Northwestern University Evanston IL
    Biomedical Engineering
  • FI Wu
    Northwestern University Evanston IL
    Biomedical Engineering
  • K Shafer
    Biomedical Engineering Department
    Northwestern University Evanston IL
  • R Van Duyne
    Chemistry Department
    Northwestern University Evanston IL
  • Footnotes
    Commercial Relationships   M.R. Glucksberg, None; F.I. Wu, None; K. Shafer, None; R. Van Duyne, None. Grant Identification: Support: NIH grants EY13002-01, EY09714 and EY13015.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 4378. doi:
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    • Get Citation

      MR Glucksberg, FI Wu, K Shafer, R Van Duyne; Novel Nanoparticles For Contrast Enhancement Optical Coherence Tomography In Doppler . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4378.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose: To devise and characterize a biologically compatible contrast enhancing agent for Optical Coherence Tomography Methods: Degeneration of the choriocapillaris has been found in postmortem histologic analysis of choroids in patients afflicted with diabetes and age-related macular degeneration. Laser Doppler flowmetry has been successfully used to measure relative flow in the choroid in animals and humans, however, due to its poor spatial resolution use is limited to the foveal region which lack retinal vessels. Color Doppler Optical Coherence Tomography has the potential to obtain spatially resolved measurements of blood velocity in the eye’s choroidal circulation, but satisfactory velocity resolution in animal models remains elusive because of inherently low contrast across the retinal and choroid. Here we used a class of high-aspect ratio, 70 nm long. Highly scattering silver nanocrystals, as contrast agents in micro-etched flow phantoms with geometries based on the choroidal circulations mammalian eyes. OCT scans were taken before and after injection of the nanospheres. Results: Tomographic velocity images of the flow phantoms show marked enhancement when the flow contains the nano-particles. Contrast in relation to non-enhanced blood and surrounding material may be increased and relative mean velocity measurements show improved contrast and increased signal-to-noise ratio in the Doppler measurement. Conclusion: Nanoparicles, if they can be tolerated in vivo, have the potential to be used as contrast agents in Doppler OCT measurements in the eye.

Keywords: 345 choroid • 331 blood supply • 431 imaging/image analysis: non-clinical 

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