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V Robredo, L Llamas, A Alcala, F Graue; A Proliferative Vitreorretinopathy Experimental Model in Adult Pigmented Rabbits with 100% of Efectiveness . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4490.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To induce Proliferative Vitreoretinopathy (PVR) in pigmented adult rabbits trying to reproduce what happens in the hispanic population obtaining fast results (less than one month) without using exogenous cells, but activating endogenous factors just as happens in the natural evolution of the disease . Methods: PVR was induced in the right eye of 20 rabbits using cryotherapy, vitrectomy, 180-200° retinotomy and an injection of autologous platelet-rich plasma. The healthy left eye was used as control. PVR developed 28 days after procedure; the contractile membranes and the remaining RPE were obtained from the right eyes, and normal RPE cells were obtained from the left eyes. A histopathologic study was done in the healthy and the afected eyes to demonstrate the development of PVR. Results: In 100% of the rabbits PVR was induced in a mean of 28 days after the procedure and the histopathologic study showed the presence of fibroblasts, pigmented epithelium cells ( PRE) that stained positive to actin with less pigment than normal cells. Conclusion: The rabbit model for PVR is suitable for studying the development of PVR in a short period of time allowing the availability of materials for biochemical and pharmacological studies, which are the basis to disclose the pathogenesis of PVR.
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