December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
The Role of Peanut Lectin in Human Retinal Pigment Epithelial Cell De-Differentiation and Proliferation
DL Kent; C Sheridan; H Tomkinson; J Pearlman; P Hiscott
Author Affiliations & Notes
  • DL Kent
    Unit of Ophthalmology University of Liverpool Liverpool United Kingdom
  • C Sheridan
    Unit of Ophthalmology University of Liverpool Liverpool United Kingdom
  • H Tomkinson
    Unit of Ophthalmology University of Liverpool Liverpool United Kingdom
  • J Pearlman
    The Departments of Ophthalmology and Neuroscience The Johns Hopkins University School of Medicine Baltimore MD
  • P Hiscott
    Unit of Ophthalmology University of Liverpool Liverpool United Kingdom
  • Footnotes
    Commercial Relationships   D.L. Kent, None; C. Sheridan, None; H. Tomkinson, None; J. Pearlman, None; P. Hiscott, None. Grant Identification: Support: British Council for Prevention of Blindness Grant R1186/2
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 4514. doi:
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      DL Kent, C Sheridan, H Tomkinson, J Pearlman, P Hiscott; The Role of Peanut Lectin in Human Retinal Pigment Epithelial Cell De-Differentiation and Proliferation . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4514.

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Abstract

Abstract: : Purpose:De-differentiation of the retinal pigment epithelium (RPE) plays a pivotal role in such diverse retinal conditions as proliferative vitreoretinopathy in retinal detachment and disciform scaring in choroidal neovascularization. We investigated the role of glycoconjugate expression in human RPE de-differentiation using peanut lectin (PNL) from Arachis hypogaea. Methods:PNL immunohistochemistry of whole eyes, epiretinal membranes (ERMs) and HRPE cell cultures was performed. A Flouorescein isothiocyanate (FITC)-labelled PNL time course assay of binding to RPE cells was also performed. The effect of PNL on RPE proliferation was studied using a bromodeoxyuridine (BRDU) assay together with cytotoxicity analysis. Results:PNL binds RPE in ERMs but not the normal RPE monolayer. In vitro studies demonstrated that PNL bound de-differentiated RPE in a time-dependent manner and this binding caused a dose-dependent inhibition of RPE proliferation that was non-cytotoxic. Conclusion:PNL may have a role in the modification of anomalous RPE behaviour in a variety of retinal diseases characterized by aberrant wound healing.

Keywords: 567 retinal pigment epithelium • 631 wound healing • 421 glycoconjugates/glycoproteins 
© 2002, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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