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JB Reed, JE Kalns, K Batey, R Lane, J Jumper; DNA Damage but not Caspase-3 Activation or Cellular Proliferation is Associated with Retinal Laser Injury . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4526.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To study the biochemical effects of retinal laser injury in areas remote from direct laser exposure. Methods: 4-7 week old Sus scrofa pigs received retinal diode laser burns at varying time intervals between 0 and 24 hours using a precise time-to-fixation technique. Areas of timed retinal laser lesions were identified histologically and immunocytochemical staining for caspase-3, proliferating cell nuclear antigen (PCNA) and the Kenlow fragment of DNA polymerase was performed. Results: By 24 hours after laser injury, DNA damage is abundant in the outer retinal layers and decreases as a function of distance from the lesion center. The absence of activated caspase-3 positive cells and apoptotic morphology suggests that cellular necrosis predominates in the first 24 hours after laser injury. Similarly, there was no evidence of early cellular proliferation. Conclusion: Early in the course of a retinal laser lesion in the porcine model, DNA damage is present without evidence of apoptosis or retinal remodeling.
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