December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Analysis Of The Expression Of NORPEG Protein In Retinal Pigment Epithelium And Other Ocular Tissues
R Kutty; RN Fariss; S Chen; W Samuel; T Duncan; CC Bridges; A El-Sherbeny; SB Smith; B Wiggert
Author Affiliations & Notes
  • R Kutty
    National Eye Institute National Institutes of Health Bethesda MD
  • RN Fariss
    National Eye Institute National Institutes of Health Bethesda MD
  • S Chen
    National Eye Institute National Institutes of Health Bethesda MD
  • W Samuel
    National Eye Institute National Institutes of Health Bethesda MD
  • T Duncan
    National Eye Institute National Institutes of Health Bethesda MD
  • CC Bridges
    Departments of Cellular Biology and Anatomy and Ophthalmology Medical College of Georgia Augusta GA
  • A El-Sherbeny
    Departments of Cellular Biology and Anatomy and Ophthalmology Medical College of Georgia Augusta GA
  • SB Smith
    Departments of Cellular Biology and Anatomy and Ophthalmology Medical College of Georgia Augusta GA
  • B Wiggert
    National Eye Institute National Institutes of Health Bethesda MD
  • Footnotes
    Commercial Relationships   R. Kutty, None; R.N. Fariss, None; S. Chen, None; W. Samuel, None; T. Duncan, None; C.C. Bridges, None; A. El-Sherbeny, None; S.B. Smith, None; B. Wiggert, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 4551. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Analysis Of The Expression Of NORPEG Protein In Retinal Pigment Epithelium And Other Ocular Tissues
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      R Kutty, RN Fariss, S Chen, W Samuel, T Duncan, CC Bridges, A El-Sherbeny, SB Smith, B Wiggert; Analysis Of The Expression Of NORPEG Protein In Retinal Pigment Epithelium And Other Ocular Tissues . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4551.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Abstract: : Purpose: NORPEG, a novel gene that we characterized from human retinal pigment epithelial (RPE) cells, encodes a putative 980 amino acid residue cytoskeletal protein containing six ankyrin repeats and a long coiled-coil domain (Kutty et al., J. Biol. Chem. 276: 2831, 2001). The expression of NORPEG protein in retinal pigment epithelium and other ocular tissues was analyzed in order to gain an insight into its functional role. Methods: Antibodies were raised in rabbits against synthetic peptides designed from the amino acid sequence of NORPEG protein. The antibody preparations were characterized using Western immunoblot analysis and then employed for immunohistochemical analysis of mouse and monkey eye sections. Results: In mouse eye, intense NORPEG immunoreactivity was detected in RPE, ganglion cell layer, Müller cell fibres, lens epithelium, corneal epithelium and ciliary body. Immunostaining of monkey retina also showed NORPEG expression in RPE, ganglion cell layer and Müller cell fibres. Conclusion: NORPEG protein is expressed in several ocular tissues, especially those containing epithelial cell types. The immunolocalization and structural characteristics of NORPEG suggest that this protein may play an important role in cell structure and function in several ocular tissues.

Keywords: 567 retinal pigment epithelium • 527 protein structure/function • 434 immunohistochemistry 
© 2002, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept