December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Examination Of Individual RPE Lipofuscin Granules As A Function Of Donor Age Using Optical And Spatial Microscopy
JD Simon; NM Haralampus-Grynaviski; LE Lamb; M Rozanowska; T Sarna; JM Burke
Author Affiliations & Notes
  • JD Simon
    Chemistry Biochemistry and Ophthalmology
    Duke University Durham NC
  • NM Haralampus-Grynaviski
    Chemistry
    Duke University Durham NC
  • LE Lamb
    Chemistry
    Duke University Durham NC
  • M Rozanowska
    Biophysics Jagiellonian University Krakow Poland
  • T Sarna
    Biophysics Jagiellonian University Krakow Poland
  • JM Burke
    Ophthalmology Medical College of Wisconsin Milwaukee WI
  • Footnotes
    Commercial Relationships   J.D. Simon, None; N.M. Haralampus-Grynaviski, None; L.E. Lamb, None; M. Rozanowska, None; T. Sarna, None; J.M. Burke, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 4555. doi:
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      JD Simon, NM Haralampus-Grynaviski, LE Lamb, M Rozanowska, T Sarna, JM Burke; Examination Of Individual RPE Lipofuscin Granules As A Function Of Donor Age Using Optical And Spatial Microscopy . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4555.

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Abstract

Abstract: : Purpose: To determine spectral and spatial properties of lipofuscin granules extracted from RPE cells of individual human donor eyes of different ages. Methods: Lipofuscin granules were isolated from the RPE cells using published procedures. The individual granules were characterized by atomic force and spectrally-resolved confocal fluorescence microscopies. Results: A broad distribution in the emission maximum of the spectra from individual lipofuscin granules isolated from single donors is observed. The average spectrum is in good agreement with previously published results from pooled samples. The distribution of emission maxima broadens with increased age of the donor eye. Conclusion: The distribution of fluorophores in lipofuscin varies among individual granules from single donors and differs for different aged eyes.

Keywords: 308 age-related macular degeneration • 567 retinal pigment epithelium • 471 microscopy: confocal/tunneling 
© 2002, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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