December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Continuous Retinyl Ester Accumulation In Rpe65 Knockout Mice
Author Affiliations & Notes
  • GP Moiseyev
    Department of Ophthalmology Medical University of South Carolina Charleston SC
  • P Goletz
    Department of Ophthalmology Medical University of South Carolina Charleston SC
  • AT C Tsin
    University of Texas San Antonio TX
  • TM Redmond
    LRCMB National Eye Institute NIH Bethesda MD
  • RK Crouch
    Department of Ophthalmology Medical University of South Carolina Charleston SC
  • J-X Ma
    Department of Ophthalmology Medical University of South Carolina Charleston SC
  • Footnotes
    Commercial Relationships   G.P. Moiseyev, None; P. Goletz, None; A.T.C. Tsin, None; T.M. Redmond, None; R.K. Crouch, None; J. Ma, None. Grant Identification: Support: NIH Grants EY12231 and EY0439 and RPB
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 4559. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      GP Moiseyev, P Goletz, AT C Tsin, TM Redmond, RK Crouch, J-X Ma; Continuous Retinyl Ester Accumulation In Rpe65 Knockout Mice . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4559.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: RPE65 knockout (Rpe65-/-) mice generate only minimal amounts of 11-cis retinoids and have elevated levels of all-trans retinyl esters in the retinal pigment epithelium (RPE). The aim of this study was to follow the dynamics of retinyl ester accumulation with age in the RPE65-/- RPE and to understand the possible reasons for overaccumulation of the retinyls esters. Methods: Retinyl esters were extracted and analyzed by HPLC. Expression levels of the proteins involved in visual cycle retinoids metabolism were determined by Western blots. Lecithin retinyl acyltransferase (LRAT) , isomerase and retinyl ester hydrolase (REH) activities were measured in the RPE of wild-type (wt) and Rpe65-/- mice. Results: In wt mice, retinyl ester levels increased with age reaching a plateau at 8 weeks of age. In the RPE65-/- mice, all-trans retinyl esters continuously increased during the entire age analyzed reaching a level over 20-fold higher than in the wt animals at 9 mo. The composition of retinyl esters is similar in wt and Rpe65-/ mice. Wt and RPE65-/- mice have similar LRAT and 11-cis REH activities whereas all-trans REH activity was shown to be reduced by 4-fold in RPE65-/- mice. Conclusion: We conclude that the lack of balance between esterification and hydrolysis may contribute, at least partially, to the continuous accumulation of the all-trans retinyl esters in the RPE of RPE65-/- mice. Supported by NIH EY12231 and EY0439 and RPB.

Keywords: 567 retinal pigment epithelium • 399 enzymes/enzyme inhibitors • 316 animal model 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×