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D Yang, A Swaminathan, BA Hughes; Expression of Inwardly Rectifying Potassium Channel Subtypes in Native Bovine Retinal Pigment Epithelium . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4568.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Previously, we demonstrated that the inwardly rectifying K+ (Kir) channel Kir7.1 is highly expressed in the apical membrane of bovine RPE. Studies on rat RPE by other groups suggested that in addition to Kir7.1, the RPE may also express Kir4.1 and Kir6.2 channel subunits. The purpose of the present study was to determine whether these and other Kir channel subunits are expressed in native bovine RPE. Methods: RT-PCR and northern blot analyses were used to detect the expression of Kir channel subunits in native bovine RPE and neural retina. The identities of PCR products were confirmed by sequencing. Results: RT-PCR analysis of total RNA revealed that Kir2.1, Kir6.1, and Kir7.1 channel subunits are expressed in both RPE and neural retina, whereas Kir4.1 and Kir6.2 transcripts are present only in neural retina. In northern blots of poly (A)+ RNA or total RNA, Kir2.1 and Kir4.1 probes hybridized to appropriately sized transcripts in neural retina but not in the RPE. The Kir7.1 probe hybridized to a major transcript at 1.5 kb in both the RPE and neural retina, but the expression levels in the RPE were significantly greater. Conclusion: The results support the idea that Kir7.1 is the major Kir channel subunit expressed in bovine RPE. Kir2.1 and Kir6.1 may also be expressed, albeit at lower levels. In contrast, no expression of Kir4.1 and Kir6.2 was detected in bovine RPE. These results suggest an expression pattern of Kir channel subunits in bovine RPE that is significantly different from that in the rat.
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